PMID- 9530331
OWN - NLM
STAT- MEDLINE
DCOM- 19980408
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 102
IP  - 1
DP  - 1998 Apr 1
TI  - Fluorescence in situ hybridization analysis of single-cell trisomies for 
      determination of clonality.
PG  - 1-5
AB  - Conflicting data currently exist pertaining to the significance of single-cell 
      trisomies found in a 20-cell cytogenetic analysis of hematologic malignancies. In 
      order to determine the clonality of the numerical abnormalities found in these 
      cases, we performed a retrospective study on a cohort of patients previously 
      analyzed by GTG-banding at Rhode Island Hospital, from July 1, 1990 to November 
      30, 1996. Fluorescence in situ hybridization (FISH) was performed using 
      chromosome enumeration probes on previously fixed bone marrow and, in some cases, 
      peripheral blood slides from patients identified to have single-cell trisomies of 
      selected chromosomes in 20-cell routine GTG-banded analyses. We seek to determine 
      whether the single cell trisomies represent random nonclonal events or "the tip 
      of an iceberg." The results of the present study indicate that a single unifying 
      answer to the above question does not exist. While some cases are apparently 
      random events as predicted by chance, other cases appear to represent "the tip of 
      an iceberg." It is therefore important for cancer cytogeneticists to interpret 
      the results of each patient on a case-by-case basis and to formulate the most 
      optimal strategy for follow-up in the particular case under study.
FAU - Mark, H F
AU  - Mark HF
AD  - Laboratory of Cytogenetics, FISH and Genotoxicology, Rhode Island Hospital, 
      Providence 02903, USA.
FAU - Rehan, J
AU  - Rehan J
FAU - Mark, S
AU  - Mark S
FAU - Santoro, K
AU  - Santoro K
FAU - Zolnierz, K
AU  - Zolnierz K
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
SB  - IM
CIN - Cancer Genet Cytogenet. 1999 Sep;113(2):193-4. PMID: 10484992
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Clone Cells
MH  - Cohort Studies
MH  - Female
MH  - Hematologic Neoplasms/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Middle Aged
MH  - Retrospective Studies
MH  - *Trisomy
EDAT- 1998/04/08 00:00
MHDA- 1998/04/08 00:01
CRDT- 1998/04/08 00:00
PHST- 1998/04/08 00:00 [pubmed]
PHST- 1998/04/08 00:01 [medline]
PHST- 1998/04/08 00:00 [entrez]
AID - S0165460897002690 [pii]
AID - 10.1016/s0165-4608(97)00269-0 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 1998 Apr 1;102(1):1-5. doi: 
      10.1016/s0165-4608(97)00269-0.