PMID- 9531967
OWN - NLM
STAT- MEDLINE
DCOM- 19980421
LR  - 20190914
IS  - 0903-4641 (Print)
IS  - 0903-4641 (Linking)
VI  - 106
IP  - 2
DP  - 1998 Feb
TI  - Viremia in chronic hepatitis C patients evaluated by the Amplicor RT-PCR, a 
      nested RT-PCR, and transaminase levels.
PG  - 334-8
AB  - A commercially available kit, Amplicor, was compared with a locally developed 
      nested reverse-transcriptase (RT) PCR for qualitative detection of HCV-RNA. 
      Sixty-one serum samples from sixty-one patients with liver disease, and 60 
      samples from 60 hemophiliacs without symptoms, but known to have been heavily 
      exposed to hepatitis C virus, were investigated. There was a high degree of 
      concordance between the two diagnostic tests (97%), the Amplicor kit being 
      slightly more sensitive than the in-house PCR, when evaluated using serial 
      dilutions of samples showing discrepant results. The relationship between viremia 
      and abnormal ALT levels was studied in the two groups of patients. Among those 
      with chronic liver disease, 8.3% of patients with viremia had normal ALT levels, 
      whereas transaminases were normal in 20% of hemophiliacs with viremia. This 
      points to ALT as being a poor marker of ongoing viral replication.
FAU - Laursen, A L
AU  - Laursen AL
AD  - Department of Infectious Diseases, Marselisborg Hospital, University of Aarhus, 
      Denmark.
FAU - Ingerslev, J
AU  - Ingerslev J
FAU - Andersen, P L
AU  - Andersen PL
FAU - Ostergaard, L
AU  - Ostergaard L
LA  - eng
PT  - Journal Article
PL  - Denmark
TA  - APMIS
JT  - APMIS : acta pathologica, microbiologica, et immunologica Scandinavica
JID - 8803400
RN  - 0 (Reagent Kits, Diagnostic)
RN  - EC 2.6.1.2 (Alanine Transaminase)
RN  - EC 2.7.7.49 (RNA-Directed DNA Polymerase)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Alanine Transaminase/*blood
MH  - Female
MH  - Hepacivirus/*genetics
MH  - Hepatitis C, Chronic/diagnosis/*virology
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Polymerase Chain Reaction/*methods
MH  - RNA-Directed DNA Polymerase
MH  - Reagent Kits, Diagnostic
MH  - Viremia/diagnosis/*virology
EDAT- 1998/04/09 00:00
MHDA- 1998/04/09 00:01
CRDT- 1998/04/09 00:00
PHST- 1998/04/09 00:00 [pubmed]
PHST- 1998/04/09 00:01 [medline]
PHST- 1998/04/09 00:00 [entrez]
AID - 10.1111/j.1699-0463.1998.tb01354.x [doi]
PST - ppublish
SO  - APMIS. 1998 Feb;106(2):334-8. doi: 10.1111/j.1699-0463.1998.tb01354.x.