PMID- 9533015
OWN - NLM
STAT- MEDLINE
DCOM- 19980422
LR  - 20180214
IS  - 0301-0171 (Print)
IS  - 0301-0171 (Linking)
VI  - 79
IP  - 1-2
DP  - 1997
TI  - Construction of two near-kilobase resolution restriction maps of the 5' 
      regulatory region of the human apolipoprotein B gene by quantitative DNA fiber 
      mapping (QDFM).
PG  - 64-70
AB  - Quantitative DNA fiber mapping (QDFM) is a high-resolution technique for physical 
      mapping of DNA. The method is based on hybridization of fluorescently labeled DNA 
      probes to individual DNA molecules stretched on a chemically modified glass 
      surface. We now demonstrate and validate a rapid QDFM-based approach for the 
      mapping of multiple restriction sites and precise localization of restriction 
      fragments in large genomic clones. Restriction fragments of a 70-kb P1 clone 
      (P1-70) containing the 5' region of the human apolipo-protein B gene (APOB) were 
      subcloned and mapped along straightened P1-70 DNA molecules. Multicolor 
      fluorescence in situ hybridization (FISH) and digital image analysis allowed us 
      to rapidly position 29 restriction fragments, ranging in size from 0.5 kb to 8 
      kb, and to map 43 restriction sites. The restriction map obtained by QDFM was in 
      excellent agreement with information obtained by RecA-assisted restriction 
      endonuclease (RARE) cleavage, long-range PCR, and DNA sequence analyses of the 
      P1-70 clone. These data demonstrate that QDFM is a rapid, reliable method for 
      detailed restriction site-mapping of large DNA clones.
FAU - Duell, T
AU  - Duell T
AD  - Life Sciences Division, Lawrence Berkeley National Laboratory, CA 94720, USA.
FAU - Nielsen, L B
AU  - Nielsen LB
FAU - Jones, A
AU  - Jones A
FAU - Young, S G
AU  - Young SG
FAU - Weier, H U
AU  - Weier HU
LA  - eng
GR  - HL47660/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Switzerland
TA  - Cytogenet Cell Genet
JT  - Cytogenetics and cell genetics
JID - 0367735
RN  - 0 (Apolipoproteins B)
RN  - 0 (Oligonucleotide Probes)
RN  - EC 2.7.7.- (Rec A Recombinases)
RN  - EC 3.1.21.- (Deoxyribonuclease EcoRI)
RN  - EC 3.1.21.- (Deoxyribonuclease HindIII)
SB  - IM
MH  - Apolipoproteins B/*genetics
MH  - Chromosomes, Artificial, Yeast
MH  - Deoxyribonuclease EcoRI
MH  - Deoxyribonuclease HindIII
MH  - Electrophoresis
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Oligonucleotide Probes
MH  - Polymerase Chain Reaction
MH  - Rec A Recombinases
MH  - Regulatory Sequences, Nucleic Acid/*genetics
MH  - Restriction Mapping/*methods
MH  - Sequence Analysis, DNA
EDAT- 1997/01/01 00:00
MHDA- 1998/04/09 00:01
CRDT- 1997/01/01 00:00
PHST- 1997/01/01 00:00 [pubmed]
PHST- 1998/04/09 00:01 [medline]
PHST- 1997/01/01 00:00 [entrez]
AID - 10.1159/000134685 [doi]
PST - ppublish
SO  - Cytogenet Cell Genet. 1997;79(1-2):64-70. doi: 10.1159/000134685.