PMID- 9533550
OWN - NLM
STAT- MEDLINE
DCOM- 19980528
LR  - 20101118
IS  - 1078-0432 (Print)
IS  - 1078-0432 (Linking)
VI  - 4
IP  - 3
DP  - 1998 Mar
TI  - Lineage-negative human leukocyte antigen-DR+ cells with the phenotype of 
      undifferentiated dendritic cells in patients with carcinoma of the abdomen and 
      pelvis.
PG  - 799-809
AB  - The characteristics of antigen-presenting cells in carcinomas that involve the 
      abdominopelvic cavity are unknown. Dendritic cells, a population of 
      antigen-presenting cells, have been identified as lineage-negative human 
      leukocyte antigen (HLA)-DR+ cells by two-color flow cytometry. We used this 
      criterion to study the putative dendritic cells in ascites from 25 patients with 
      peritoneal carcinomatosis. The mean proportion +/- SD of lineage-negative HLA-DR+ 
      cells in ascites was 3.1 +/- 4.6% (range, 0.05-17.3%). Most lineage-negative 
      HLA-DR+ cells expressed CD45RA or CD4 antigens. Dendritic cells had low 
      proportions of CD80, CD11c, CD45RO, and CD58, suggesting that they were of low 
      maturity. The proportion of lineage-negative HLA-DR+ cells in ascites of seven 
      patients was significantly higher than the proportion in peripheral blood from 
      the identical patients (4.5 +/- 5.7 versus 0.5 +/- 0.4; P < 0.05). In paired 
      specimens of ascites and peripheral blood, the proportion of lineage-negative 
      HLA-DR+ cells that coexpressed CD86 or CD58 was significantly lower in ascites 
      than in peripheral blood, whereas a higher proportion of lineage-negative HLA-DR+ 
      cells in ascites expressed CD4. Relative fluorescence intensity of HLA-DR+ was 
      also lower in dendritic cells from ascites and blood from patients with 
      carcinomatosis than it was in blood from normal donors. As an indicator of 
      macrophage activation, the concentration of neopterin in ascitic fluid correlated 
      negatively with the numbers of lineage-negative HLA-DR+ cells in ascites 
      (Spearman correlation coefficient, -0.44; P = 0.05) correlated positively with 
      the concentration of interleukin 10 in ascitic fluid (Spearman correlation 
      coefficient, -0.40; P = 0.05). IFN-gamma and tumor necrosis factor alpha were 
      also not detected. These findings suggest that certain factors associated with 
      the tumor microenvironment might influence the number of these dendritic cells 
      and their expression of function-associated markers.
FAU - Melichar, B
AU  - Melichar B
AD  - Department of Gynecologic Oncology, The University of Texas M. D. Anderson Cancer 
      Center, Houston 77030, USA.
FAU - Savary, C
AU  - Savary C
FAU - Kudelka, A P
AU  - Kudelka AP
FAU - Verschraegen, C
AU  - Verschraegen C
FAU - Kavanagh, J J
AU  - Kavanagh JJ
FAU - Edwards, C L
AU  - Edwards CL
FAU - Platsoucas, C D
AU  - Platsoucas CD
FAU - Freedman, R S
AU  - Freedman RS
LA  - eng
GR  - MO1-RR02558/RR/NCRR NIH HHS/United States
GR  - R01-CA64943/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Clin Cancer Res
JT  - Clinical cancer research : an official journal of the American Association for 
      Cancer Research
JID - 9502500
RN  - 0 (Antigens, CD)
RN  - 0 (HLA-DR Antigens)
SB  - IM
MH  - Abdominal Neoplasms/*immunology/pathology
MH  - Adult
MH  - Aged
MH  - Antigens, CD/*analysis
MH  - Ascites/immunology
MH  - Dendritic Cells/*immunology/pathology
MH  - Female
MH  - Flow Cytometry
MH  - HLA-DR Antigens/*analysis
MH  - Humans
MH  - Immunophenotyping
MH  - Middle Aged
MH  - Ovarian Neoplasms/immunology/pathology
MH  - Pelvic Neoplasms/*immunology/pathology
MH  - Peritoneal Neoplasms/*immunology/pathology
MH  - Reference Values
MH  - Therapeutic Irrigation
EDAT- 1998/05/14 00:00
MHDA- 1998/05/14 00:01
CRDT- 1998/05/14 00:00
PHST- 1998/05/14 00:00 [pubmed]
PHST- 1998/05/14 00:01 [medline]
PHST- 1998/05/14 00:00 [entrez]
PST - ppublish
SO  - Clin Cancer Res. 1998 Mar;4(3):799-809.