PMID- 9533613
OWN - NLM
STAT- MEDLINE
DCOM- 19980601
LR  - 20191102
IS  - 1064-1963 (Print)
IS  - 1064-1963 (Linking)
VI  - 20
IP  - 2
DP  - 1998 Feb
TI  - Mapping of candidate genes for hypertension by fluorescence in situ hybridization 
      on the genome of transgenic rats and mice.
PG  - 185-204
AB  - Transgenic animals are new and important models for the study of candidate genes 
      in hypertension research as well as in other fields of medicine. For detailed 
      genetic characterization of the transgenic animals, and to account for the 
      symptoms arising from the insertion of transgenes in the genome, it is essential 
      to identify these insertion sites. In this study, the insertion sites of the 
      transgenes of candidate genes for hypertension were identified by fluorescence in 
      situ hybridization (FISH) after G-banding of the chromosomes in transgenic rats 
      and mice. This technique combines high resolution G-banding and fluorescence in 
      situ hybridization for the mapping of four different candidate genes in six 
      different transgenic rats as well as three different mouse transgenic lines. The 
      presented results will help to draw conclusions about the influence of the 
      respective integration site on transgene expression.
FAU - Schmidt, C
AU  - Schmidt C
AD  - Institute for Human Genetics, Heidelberg, Germany.
FAU - Ganten, D
AU  - Ganten D
FAU - Klenk, A
AU  - Klenk A
FAU - Buselmaier, W
AU  - Buselmaier W
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Clin Exp Hypertens
JT  - Clinical and experimental hypertension (New York, N.Y. : 1993)
JID - 9305929
RN  - 0 (Endothelin-2)
RN  - 11002-13-4 (Angiotensinogen)
RN  - EC 3.4.23.15 (Renin)
SB  - IM
MH  - Angiotensinogen/genetics/physiology
MH  - Animals
MH  - Animals, Genetically Modified
MH  - Chromosome Banding
MH  - *Chromosome Mapping
MH  - Disease Models, Animal
MH  - Endothelin-2/genetics/physiology
MH  - Humans
MH  - Hypertension/*genetics/physiopathology
MH  - In Situ Hybridization, Fluorescence
MH  - Mice
MH  - Mice, Transgenic
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Renin/genetics/physiology
EDAT- 1998/04/09 00:00
MHDA- 1998/04/09 00:01
CRDT- 1998/04/09 00:00
PHST- 1998/04/09 00:00 [pubmed]
PHST- 1998/04/09 00:01 [medline]
PHST- 1998/04/09 00:00 [entrez]
AID - 10.3109/10641969809053214 [doi]
PST - ppublish
SO  - Clin Exp Hypertens. 1998 Feb;20(2):185-204. doi: 10.3109/10641969809053214.