PMID- 9536232
OWN - NLM
STAT- MEDLINE
DCOM- 19980423
LR  - 20190704
IS  - 0007-0963 (Print)
IS  - 0007-0963 (Linking)
VI  - 138
IP  - 1
DP  - 1998 Jan
TI  - Counterimmunoelectrophoresis, ELISA and immunoblotting detection of anti-Ro/SSA 
      antibodies in subacute cutaneous lupus erythematosus. A comparative study.
PG  - 114-7
AB  - Patients with subacute cutaneous lupus erythematosus (SCLE) have circulating 
      antibodies to Ro/SSA directed to two antigenically distinct ribonucleoproteins of 
      60 kDa and 52 kDa. Three laboratory tests may be used to detect anti-Ro/SSA 
      antibodies: counterimmunoelectrophoresis (CIE), enzyme-linked immunosorbent assay 
      (ELISA) and immunoblotting (IB). Their relative efficacy and clinical 
      correlations were ascertained. We determined anti-Ro/SSA antibodies with CIE, 
      with two different ELISA methods (ELISA 1 and 2) and with IB in 29 SCLE patients. 
      Anti-52 kDa and -60 kDa Ro/SSA antibodies were also assayed with IB. In addition, 
      we determined antinuclear antibodies with indirect immunofluorescence, anti-Sm, 
      anti-RNP, anti-La/SSB and anti-Ro/SSA antibodies with CIE and ELISA, and 
      anti-nDNA and cardiolipin antibodies using an ELISA method. CIE detected 
      anti-Ro/SSA antibodies in 22 patients while ELISA 1 and 2 did so in 17 and 18 
      patients, respectively. In five patients, IB revealed a reactivity to 60 kDa 
      polypeptides and in two, a reactivity to 52 kDa polypeptides. Of these seven 
      patients, four had a myocardial infarction. Of these, two reacted to the 52 kDa 
      antigen and two to the 60 kDa antigen. A combination of techniques was often 
      needed to detect all specificities. ELISA proved to be very specific and 
      sensitive. The IB technique detected a group of patients with myocardial 
      infarction. A case-control study is needed to confirm the data of cardiac 
      involvement.
FAU - Parodi, A
AU  - Parodi A
AD  - Department of Dermatology, University of Genoa, Italy.
FAU - Drosera, M
AU  - Drosera M
FAU - Barbieri, L
AU  - Barbieri L
FAU - Rebora, A
AU  - Rebora A
LA  - eng
PT  - Clinical Trial
PT  - Comparative Study
PT  - Journal Article
PL  - England
TA  - Br J Dermatol
JT  - The British journal of dermatology
JID - 0004041
RN  - 0 (Antibodies, Antinuclear)
RN  - 0 (Biomarkers)
RN  - 0 (SS-A antibodies)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Antibodies, Antinuclear/*analysis
MH  - Antibody Specificity
MH  - Biomarkers
MH  - Counterimmunoelectrophoresis
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Humans
MH  - Immunoblotting
MH  - Lupus Erythematosus, Cutaneous/*immunology
MH  - Male
MH  - Middle Aged
MH  - Myocardial Infarction/immunology
MH  - Sensitivity and Specificity
EDAT- 1998/04/16 00:00
MHDA- 1998/04/16 00:01
CRDT- 1998/04/16 00:00
PHST- 1998/04/16 00:00 [pubmed]
PHST- 1998/04/16 00:01 [medline]
PHST- 1998/04/16 00:00 [entrez]
AID - 10.1046/j.1365-2133.1998.02035.x [doi]
PST - ppublish
SO  - Br J Dermatol. 1998 Jan;138(1):114-7. doi: 10.1046/j.1365-2133.1998.02035.x.