PMID- 9536978
OWN - NLM
STAT- MEDLINE
DCOM- 19980421
LR  - 20101118
IS  - 0022-4804 (Print)
IS  - 0022-4804 (Linking)
VI  - 74
IP  - 1
DP  - 1998 Jan
TI  - Proteoglycan gene expression is decreased in abdominal aortic aneurysms.
PG  - 76-80
AB  - BACKGROUND: Abdominal aortic aneurysms (AAA) are characterized by both increases 
      in proteolysis and changes in the biosynthesis of the extracellular matrix (ECM) 
      proteins. Proteoglycans are important components of the ECM, particularly the 
      small proteoglycans, biglycan and decorin. Biglycan and decorin regulate cell 
      proliferation and collagen assembly. Therefore, the purpose of this study is to 
      quantify the levels of mRNA for biglycan and decorin in normal aorta (Na) and 
      AAA. MATERIALS AND METHODS: Northern blot hybridization and competitive 
      polymerase chain reaction using gene-specific external standards were used to 
      quantify mRNA levels of bigylcan and decorin in RNA derived from AAA and NA. 
      Results are expressed as a percentage of glyceraldehyde-3-phosphate dehydrogenase 
      or normalized to ribosomal RNA content and compared using the unpaired t test. 
      RESULTS: A statistically significant 15-fold decrease in biglycan mRNA expression 
      was observed in AAA compared to NA (176.9% vs 11.8%, P < 0.001). In contrast to 
      biglycan, the decorin mRNA expression is unchanged in AAA compared to NA. 
      CONCLUSIONS: The marked decrease in biglycan mRNA levels is unique to aneurysmal 
      disease of the aorta. In atherosclerosis and restenosis, biglycan expression is 
      increased in comparison with normal artery. This decrease in biglycan expression 
      may reflect important regulatory changes specific for the AAA. Furthermore, a 
      decrease in biglycan gene expression and biosynthesis could have a broad impact 
      on the physiology and matrix architecture of the aorta.
FAU - Tamarina, N A
AU  - Tamarina NA
AD  - Department of Surgery, Northwestern University Medical School, Chicago, Illinois 
      60611, USA.
FAU - Grassi, M A
AU  - Grassi MA
FAU - Johnson, D A
AU  - Johnson DA
FAU - Pearce, W H
AU  - Pearce WH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Surg Res
JT  - The Journal of surgical research
JID - 0376340
RN  - 0 (BGN protein, human)
RN  - 0 (Biglycan)
RN  - 0 (DCN protein, human)
RN  - 0 (DNA Primers)
RN  - 0 (Decorin)
RN  - 0 (Extracellular Matrix Proteins)
RN  - 0 (Proteoglycans)
RN  - 0 (RNA, Messenger)
RN  - 9007-34-5 (Collagen)
SB  - IM
MH  - Adult
MH  - Aorta, Abdominal/metabolism
MH  - Aortic Aneurysm, Abdominal/*genetics/*metabolism
MH  - Base Sequence
MH  - Biglycan
MH  - Collagen/metabolism
MH  - DNA Primers/genetics
MH  - Decorin
MH  - Extracellular Matrix Proteins
MH  - Female
MH  - *Gene Expression
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Polymerase Chain Reaction
MH  - Proteoglycans/*genetics/metabolism
MH  - RNA, Messenger/genetics/metabolism
EDAT- 1998/04/16 00:00
MHDA- 1998/04/16 00:01
CRDT- 1998/04/16 00:00
PHST- 1998/04/16 00:00 [pubmed]
PHST- 1998/04/16 00:01 [medline]
PHST- 1998/04/16 00:00 [entrez]
AID - S0022-4804(97)95201-X [pii]
AID - 10.1006/jsre.1997.5201 [doi]
PST - ppublish
SO  - J Surg Res. 1998 Jan;74(1):76-80. doi: 10.1006/jsre.1997.5201.