PMID- 9542319
OWN - NLM
STAT- MEDLINE
DCOM- 19980513
LR  - 20061115
IS  - 0390-6078 (Print)
IS  - 0390-6078 (Linking)
VI  - 83
IP  - 1
DP  - 1998 Jan
TI  - Detection and monitoring of trisomy 8 by fluorescence in situ hybridization in 
      acute myeloid leukemia: a multicentric study.
PG  - 21-6
AB  - BACKGROUND AND OBJECTIVE: The role of fluorescence in situ hybridization (FISH) 
      in the detection and monitoring of trisomy 8 (+8) in acute myelogenous leukemia 
      (AML) has not been defined exactly. This multicentric study was performed in 
      order to: i) analyze the sensitivity of interphase FISH with respect to 
      conventional chromosome analysis (CCA) in detecting +8; ii) compare the results 
      of FISH and CCA in the quantitation of the frequency of +8-positive cells; iii) 
      analyze the possible role of FISH in the cytogenetic follow-up of patients with 
      +8. DESIGN AND METHODS: One hundred and ninety-eight nonconsecutive patients with 
      a diagnosis of AML seen at five centers over a 3-year period were studied by CCA 
      and FISH with a chromosome 8-specific centromeric probe. Two hundred interphase 
      cells were scored in each test and the cut-off for the recognition of +8 was set 
      at 3%. An irrelevant pericentromeric probe was used as negative control in those 
      cases with an apparently normal karyotype and trisomy 8 in interphase cells. FISH 
      studies were conducted at diagnosis and, in 14 cases with +8, on 1.5 occasions 
      during follow-up. RESULTS: Karyotype aberrations were seen in 121 cases (61.1%), 
      with +8 being present in 38 of them (16 as the sole aberration). Interphase FISH 
      detected +8 in 37/38 cases; in a patient with 1/10 metaphases with +8, 2.3% 
      interphase cells with 3 signals were seen. Fourteen additional cases with occult 
      +8 were detected by FISH, which showed 4-22% interphase cells with three signals; 
      6 patients had an abnormal karyotype without +8, 3 had a normal karyotype, 5 had 
      no analyzable mitoses. In 24 cases with > 15 analyzable metaphases, percent 
      variations between CCA and FISH in the estimation of the size of the trisomic 
      clone ranged between 0.4% and 51%, median value 22%. Underestimation of the 
      percent of trisomy 8 by FISH occurred in all 10 cases with > 90% +8 metaphases. 
      In 7/14 cases investigated sequentially, FISH detected 5-35% trisomic cells in 
      the BM after induction therapy (4 CR, 3 PR); 4 cases relapsed with +8 at 8-15 
      months. The absence of +8 in remission marrows was documented in the remaining 7 
      cases, 4 of which relapsed at 20-32 months. INTERPRETATION AND CONCLUSIONS: It is 
      concluded that FISH was a valuable method in this multicentric study since it 
      showed greater sensitivity than CCA in detecting minor clones with +8, in 
      patients with both normal and abnormal karyotypes. The role of FISH in the 
      cytogenetic follow-up of trisomies in AML patients may be promising.
FAU - Cuneo, A
AU  - Cuneo A
AD  - Institute of Hematology, University of Ferrara, Italy.
FAU - Bigoni, R
AU  - Bigoni R
FAU - Roberti, M G
AU  - Roberti MG
FAU - Bardi, A
AU  - Bardi A
FAU - Rigolin, G M
AU  - Rigolin GM
FAU - Piva, N
AU  - Piva N
FAU - Mancini, M
AU  - Mancini M
FAU - Nanni, M
AU  - Nanni M
FAU - Alimena, G
AU  - Alimena G
FAU - Mecucci, C
AU  - Mecucci C
FAU - Matteucci, C
AU  - Matteucci C
FAU - La Starza, R
AU  - La Starza R
FAU - Bernasconi, P
AU  - Bernasconi P
FAU - Cavigliano, P
AU  - Cavigliano P
FAU - Genini, E
AU  - Genini E
FAU - Zaccaria, A
AU  - Zaccaria A
FAU - Testoni, N
AU  - Testoni N
FAU - Carboni, C
AU  - Carboni C
FAU - Castoldi, G
AU  - Castoldi G
LA  - eng
PT  - Clinical Trial
PT  - Journal Article
PT  - Multicenter Study
PT  - Research Support, Non-U.S. Gov't
PL  - Italy
TA  - Haematologica
JT  - Haematologica
JID - 0417435
SB  - IM
MH  - Acute Disease
MH  - Aged
MH  - Aged, 80 and over
MH  - *Chromosomes, Human, Pair 8
MH  - Cloning, Molecular
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Leukemia, Myeloid/*genetics
MH  - Male
MH  - Middle Aged
MH  - Remission Induction
MH  - Trisomy/*diagnosis
EDAT- 1998/05/16 00:00
MHDA- 1998/05/16 00:01
CRDT- 1998/05/16 00:00
PHST- 1998/05/16 00:00 [pubmed]
PHST- 1998/05/16 00:01 [medline]
PHST- 1998/05/16 00:00 [entrez]
PST - ppublish
SO  - Haematologica. 1998 Jan;83(1):21-6.