PMID- 9544190
OWN - NLM
STAT- MEDLINE
DCOM- 19980423
LR  - 20071114
IS  - 0893-6692 (Print)
IS  - 0893-6692 (Linking)
VI  - 31
IP  - 2
DP  - 1998
TI  - Epididymal sperm aneuploidies in three strains of rats detected by multicolor 
      fluorescence in situ hybridization.
PG  - 125-32
AB  - A multicolor fluorescence in situ hybridization (FISH) method was developed to 
      detect aneuploidy and diploidy in epididymal sperm of rats using DNA probes 
      specific for chromosomes 4 and Y. Fourteen healthy young-adult rats from three 
      strains were evaluated: inbred Fisher 344/N/ehs, outbred Sprague-Dawley, and 
      outbred WU Wistar/CPB. The hybridization efficiency of the FISH procedure was > 
      99.9%, the sex-ratio in sperm was approximately 1 as expected, and there was no 
      significant variation among two independent scorers. No significant variations 
      were detected within or among strains in the frequencies of sperm disomy for 
      chromosome 4 (1-6.5 per 10,000 cell per animal) or the Y chromosome (0-2.5 per 
      10,000 cells per animal). There was a trend toward increased variation among 
      Wistar rats. The frequencies of sperm-carrying hyper- and hypohaploidy for 
      chromosome 4 were similar, suggesting a symmetrical mechanism of chromosome gain 
      and loss during meiosis. The frequencies of Y-Y-4-4 sperm, which represent 
      genomic meiosis II errors, did not differ significantly across strains (0.1-0.7 
      per 10,000 cells per strain). This FISH method for detecting aneuploidy in rat 
      epididymal sperm provides a promising interspecies biomarker of male germ cell 
      aneuploidy and introduces the rat as an animal model for investigating the 
      heritable risk to offspring associated with paternal genotype, physiology, and 
      exposure to environmental mutagens. There appear to be no significant differences 
      among young healthy rats, mice, and men in the baseline frequencies of sperm with 
      Y chromosomal disomy, the only chromosome for which data currently exists for all 
      three species.
FAU - Lowe, X R
AU  - Lowe XR
AD  - Biology and Biotechnology Research Program, Lawrence Livermore National 
      Laboratory, CA 94550, USA.
FAU - de Stoppelaar, J M
AU  - de Stoppelaar JM
FAU - Bishop, J
AU  - Bishop J
FAU - Cassel, M
AU  - Cassel M
FAU - Hoebee, B
AU  - Hoebee B
FAU - Moore, D 2nd
AU  - Moore D 2nd
FAU - Wyrobek, A J
AU  - Wyrobek AJ
LA  - eng
GR  - IAG Y01-ES-10203-00/ES/NIEHS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Environ Mol Mutagen
JT  - Environmental and molecular mutagenesis
JID - 8800109
SB  - IM
MH  - *Aneuploidy
MH  - Animals
MH  - Cell Nucleus/chemistry
MH  - Chromosome Aberrations
MH  - Chromosome Disorders
MH  - Chromosomes/chemistry/genetics
MH  - Epididymis/cytology
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Male
MH  - Rats
MH  - Rats, Inbred F344
MH  - Rats, Sprague-Dawley
MH  - Rats, Wistar
MH  - Sex Chromosome Aberrations
MH  - Species Specificity
MH  - Spermatozoa/*physiology
MH  - Y Chromosome/chemistry/genetics
EDAT- 1998/04/17 02:02
MHDA- 2000/06/20 09:00
CRDT- 1998/04/17 02:02
PHST- 1998/04/17 02:02 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1998/04/17 02:02 [entrez]
AID - 10.1002/(SICI)1098-2280(1998)31:2<125::AID-EM4>3.0.CO;2-L [pii]
PST - ppublish
SO  - Environ Mol Mutagen. 1998;31(2):125-32.