PMID- 9544579
OWN - NLM
STAT- MEDLINE
DCOM- 19980429
LR  - 20190516
IS  - 0741-5400 (Print)
IS  - 0741-5400 (Linking)
VI  - 63
IP  - 4
DP  - 1998 Apr
TI  - Chemokine synthesis in the HSV-1-infected cornea and its suppression by 
      interleukin-10.
PG  - 486-92
AB  - Herpes simplex virus type 1 (HSV-1) infection of the murine cornea results in a 
      tissue-destructive inflammatory response. In this study we show that virus 
      infection induces the synthesis of macrophage inflammatory protein-2 (MIP-2), 
      MIP-1alpha, and monocyte chemoattractant protein-1 (MCP-1). However, only the 
      production of MIP-2 and MIP-1alpha coincided with the influx of leukocytes into 
      the cornea. IL-10 treatment markedly suppressed chemokine message and protein 
      synthesis in vivo. Local administration of IL-10 also dramatically reduced the 
      number of T cells and neutrophils migrating into the cornea and suppressed the 
      severity of corneal disease. The inflammatory response could also be suppressed 
      by the passive transfer of neutralizing antibody to MIP-1alpha but not MCP-1. We 
      conclude that local IL-10 administration can suppress chemokine synthesis, 
      thereby ameliorating corneal disease. Furthermore, our results indicate that 
      MIP-1alpha plays a major role in herpes stromal keratitis development, whereas 
      MCP-1 does not.
FAU - Tumpey, T M
AU  - Tumpey TM
AD  - Department of Microbiology and Immunology, University of South Alabama, Mobile 
      36688-0002, USA.
FAU - Cheng, H
AU  - Cheng H
FAU - Yan, X T
AU  - Yan XT
FAU - Oakes, J E
AU  - Oakes JE
FAU - Lausch, R N
AU  - Lausch RN
LA  - eng
GR  - EY07564/EY/NEI NIH HHS/United States
GR  - EY11493/EY/NEI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - J Leukoc Biol
JT  - Journal of leukocyte biology
JID - 8405628
RN  - 0 (Antibodies)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokine CCL3)
RN  - 0 (Chemokine CCL4)
RN  - 0 (Chemokine CXCL2)
RN  - 0 (Chemokines)
RN  - 0 (Macrophage Inflammatory Proteins)
RN  - 0 (Monokines)
RN  - 130068-27-8 (Interleukin-10)
SB  - IM
MH  - Animals
MH  - Antibodies/pharmacology
MH  - CD4-Positive T-Lymphocytes/cytology/drug effects
MH  - Cells, Cultured
MH  - Chemokine CCL2/immunology/physiology
MH  - Chemokine CCL3
MH  - Chemokine CCL4
MH  - Chemokine CXCL2
MH  - Chemokines/*metabolism
MH  - Chemotaxis, Leukocyte/drug effects
MH  - Cornea/drug effects/*metabolism
MH  - Female
MH  - Herpesvirus 1, Human/*immunology
MH  - Immunization, Passive
MH  - Immunohistochemistry
MH  - Interleukin-10/*pharmacology
MH  - Keratitis, Herpetic/*metabolism
MH  - Macrophage Inflammatory Proteins/immunology/physiology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Monokines/physiology
MH  - Polymerase Chain Reaction
MH  - Time Factors
EDAT- 1998/04/17 00:00
MHDA- 1998/04/17 00:01
CRDT- 1998/04/17 00:00
PHST- 1998/04/17 00:00 [pubmed]
PHST- 1998/04/17 00:01 [medline]
PHST- 1998/04/17 00:00 [entrez]
AID - 10.1002/jlb.63.4.486 [doi]
PST - ppublish
SO  - J Leukoc Biol. 1998 Apr;63(4):486-92. doi: 10.1002/jlb.63.4.486.