PMID- 9545096
OWN - NLM
STAT- MEDLINE
DCOM- 19980521
LR  - 20071114
IS  - 0148-7299 (Print)
IS  - 0148-7299 (Linking)
VI  - 76
IP  - 4
DP  - 1998 Apr 1
TI  - A multicenter investigation with interphase fluorescence in situ hybridization 
      using X- and Y-chromosome probes.
PG  - 318-26
AB  - Twenty-six laboratories used X and Y chromosome probes and the same procedures to 
      process and examine 15,600 metaphases and 49,400 interphases from Phaseolus 
      vulgaris-leucoagglutinin (PHA)-stimulated lymphocytes. In Part I, each laboratory 
      scored 50 metaphases and 200 interphases from a normal male and a normal female 
      from its own practice. In Part II, each laboratory scored 50 metaphases and 200 
      interphases on slides prepared by a central laboratory from a normal male and a 
      normal female and three mixtures of cells from the male and female. In Part III, 
      each laboratory scored 50 metaphases (in samples of 5, 10, 15, and 20) and 100 
      interphases (in samples of 5, 10, 15, 20, and 50) on new, coded slides of the 
      same specimens used in Part II. Metaphases from male specimens were scored as 
      98-99% XY with no XX cells, and 97-98% of interphases were scored as XY with 
      0.04% XX cells. Metaphases from female specimens were scored as 96-97% XX with 
      0.03% XY cells, and 94-96% of interphases were scored as XX with 0.05% XY cells. 
      Considering the data as a model for any probe used with fluorescence in situ 
      hybridization (FISH), a statistical approach assessing the impact of analytical 
      sensitivity on the numbers of observations required to assay for potential 
      mosaicisms and chimerisms is discussed. The workload associated with processing 
      slides and scoring 50 metaphases and 200 interphases using FISH averaged 27.1 and 
      28.6 minutes, respectively. This study indicates that multiple laboratories can 
      test/develop guidelines for the rapid, efficacious, and cost-effective 
      integration of FISH into clinical service.
FAU - Dewald, G
AU  - Dewald G
AD  - Mayo Clinic, Rochester, Minnesota 55905, USA.
FAU - Stallard, R
AU  - Stallard R
FAU - Al Saadi, A
AU  - Al Saadi A
FAU - Arnold, S
AU  - Arnold S
FAU - Bader, P I
AU  - Bader PI
FAU - Blough, R
AU  - Blough R
FAU - Chen, K
AU  - Chen K
FAU - Elejalde, B R
AU  - Elejalde BR
FAU - Harris, C J
AU  - Harris CJ
FAU - Higgins, R R
AU  - Higgins RR
FAU - Hoeltge, G A
AU  - Hoeltge GA
FAU - Hsu, W T
AU  - Hsu WT
FAU - Kubic, V
AU  - Kubic V
FAU - McCorquodale, D J
AU  - McCorquodale DJ
FAU - Micale, M A
AU  - Micale MA
FAU - Moore, J W
AU  - Moore JW
FAU - Phillips, R M
AU  - Phillips RM
FAU - Scheib-Wixted, S
AU  - Scheib-Wixted S
FAU - Schwartz, S
AU  - Schwartz S
FAU - Siembieda, S
AU  - Siembieda S
FAU - Strole, K
AU  - Strole K
FAU - VanTuinen, P
AU  - VanTuinen P
FAU - Vance, G H
AU  - Vance GH
FAU - Wiktor, A
AU  - Wiktor A
FAU - Zinsmeister, A
AU  - Zinsmeister A
AU  - et al.
LA  - eng
GR  - MCJ-551004-04/PHS HHS/United States
PT  - Journal Article
PT  - Multicenter Study
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Am J Med Genet
JT  - American journal of medical genetics
JID - 7708900
RN  - 0 (DNA Probes)
RN  - 0 (Phytohemagglutinins)
SB  - IM
MH  - Cytogenetics/standards
MH  - *DNA Probes
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/instrumentation/*methods
MH  - *Interphase
MH  - Laboratories/standards
MH  - Lymphocyte Activation
MH  - Lymphocytes/cytology
MH  - Male
MH  - Metaphase
MH  - Phytohemagglutinins
MH  - Quality Control
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - Workload
MH  - *X Chromosome
MH  - *Y Chromosome
EDAT- 1998/04/17 02:03
MHDA- 2000/06/20 09:00
CRDT- 1998/04/17 02:03
PHST- 1998/04/17 02:03 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1998/04/17 02:03 [entrez]
AID - 10.1002/(SICI)1096-8628(19980401)76:4<318::AID-AJMG7>3.0.CO;2-L [pii]
PST - ppublish
SO  - Am J Med Genet. 1998 Apr 1;76(4):318-26.