PMID- 9546344
OWN - NLM
STAT- MEDLINE
DCOM- 19980428
LR  - 20211203
IS  - 0002-9440 (Print)
IS  - 1525-2191 (Electronic)
IS  - 0002-9440 (Linking)
VI  - 152
IP  - 4
DP  - 1998 Apr
TI  - Regulation of uveal melanoma interconverted phenotype by hepatocyte growth 
      factor/scatter factor (HGF/SF).
PG  - 855-63
AB  - Human uveal melanoma disseminates initially and preferentially to the liver. This 
      study describes the relationship between the expression of the c-met 
      proto-oncogene (receptor for hepatocyte growth factor/scatter factor (HGF/SF)) in 
      interconverted uveal melanoma cells (co-expressing vimentin and keratin 
      intermediate filaments) and the regulation of their motogenic response to HGF/SF, 
      a key step in local invasion and targeted dissemination to the liver. Expression 
      of c-met in uveal melanoma cell lines correlates with both the appearance of an 
      interconverted phenotype and invasive ability (measured in vitro). Using 
      chemotactic checkerboard analysis, the greatest motogenic response to HGF/SF was 
      achieved by invasive, interconverted, c-met-positive uveal melanoma cells. C-met 
      was observed histologically in a uveal melanoma containing interconverted cells 
      but was absent in a tumor composed of non-interconverted cells (vimentin 
      positive/keratin negative). The c-met ligand, HGF/SF, although not expressed by 
      uveal melanoma cell lines, was localized in tissue sections of primary uveal 
      melanomas and metastatic melanoma to the liver. In the primary tumor, staining 
      for HGF/SF was most intense at the level of the choriocapillaris, a finding that 
      is significant because 1) highly remodeled neovascular loops and networks, which 
      appear in tumors likely to disseminate, can be traced to the choriocapillaris and 
      the draining vortex veins and 2) HGF/SF plays a role in tumor angiogenesis. Foci 
      of metastatic melanoma to the liver stain diffusely for HGF/SF. Regulation of the 
      uveal melanoma interconverted phenotype by HGF/SF may play an important role in 
      the dissemination of this tumor.
FAU - Hendrix, M J
AU  - Hendrix MJ
AD  - Department of Anatomy and Cell Biology, Iowa Cancer Center, The University of 
      Iowa College of Medicine, Iowa City 52242-1109, USA. mary-hendrix@uiowa.edu
FAU - Seftor, E A
AU  - Seftor EA
FAU - Seftor, R E
AU  - Seftor RE
FAU - Kirschmann, D A
AU  - Kirschmann DA
FAU - Gardner, L M
AU  - Gardner LM
FAU - Boldt, H C
AU  - Boldt HC
FAU - Meyer, M
AU  - Meyer M
FAU - Pe'er, J
AU  - Pe'er J
FAU - Folberg, R
AU  - Folberg R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Am J Pathol
JT  - The American journal of pathology
JID - 0370502
RN  - 0 (MAS1 protein, human)
RN  - 0 (Proto-Oncogene Mas)
RN  - 0 (Vimentin)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
RN  - 68238-35-7 (Keratins)
RN  - EC 2.7.10.1 (Proto-Oncogene Proteins c-met)
SB  - IM
MH  - Blotting, Northern
MH  - Cell Movement/drug effects
MH  - Hepatocyte Growth Factor/metabolism/pharmacology/*physiology
MH  - Humans
MH  - Immunohistochemistry
MH  - Keratins/metabolism
MH  - Liver Neoplasms/diagnosis/metabolism/secondary
MH  - Melanoma/*metabolism/pathology
MH  - Microscopy, Confocal
MH  - Models, Biological
MH  - Prognosis
MH  - Proto-Oncogene Mas
MH  - Proto-Oncogene Proteins c-met/metabolism
MH  - Tumor Cells, Cultured
MH  - Uveal Neoplasms/*metabolism/pathology
MH  - Vimentin/metabolism
PMC - PMC1858259
EDAT- 1998/04/18 00:00
MHDA- 1998/04/18 00:01
PMCR- 1998/10/01
CRDT- 1998/04/18 00:00
PHST- 1998/04/18 00:00 [pubmed]
PHST- 1998/04/18 00:01 [medline]
PHST- 1998/04/18 00:00 [entrez]
PHST- 1998/10/01 00:00 [pmc-release]
PST - ppublish
SO  - Am J Pathol. 1998 Apr;152(4):855-63.