PMID- 9547685
OWN - NLM
STAT- MEDLINE
DCOM- 19980611
LR  - 20190515
IS  - 1058-0468 (Print)
IS  - 1573-7330 (Electronic)
IS  - 1058-0468 (Linking)
VI  - 15
IP  - 3
DP  - 1998 Mar
TI  - Chromosome 21 detection in human oocyte fluorescence in situ hybridization: 
      possible effect of maternal age.
PG  - 105-10
AB  - PURPOSE: The purpose of this study was to evaluate, among 100 uncleaved oocytes, 
      the incidence of numerical and structural chromosome 21 and X abnormalities and 
      to analyze the influence of various factors, such as in vitro (IVF) indications, 
      follicle stimulation protocols, and women's age. METHODS: We investigated 150 
      uncleaved oocytes from 128 patients after an IVF attempt. After cytogenetic 
      analysis (Giemsa) 100 oocytes (66%) were selected for fluorescence in situ 
      hybridization (FISH). Fluorescent probes for human chromosomes X and 21 were used 
      simultaneously according to standard procedures for their hybridization and 
      detection. RESULTS AND CONCLUSIONS: We analyzed by the FISH protocol 100 
      metaphase II oocytes with 22 to 25 chromosomes. Our results demonstrate a high 
      rate of disomy for chromosome 21 in human oocytes. Among them, eight were disomic 
      (8%) and three were nullosomic (3%) for chromosome 21. Only one disomy of 
      chromosome X was noted. The various indications of IVF and the different 
      folliculogenesis stimulating protocols did not seem to influence the results but 
      suggested a correlation between the maternal age and the aneuploidy rate of 
      chromosome 21.
FAU - Benzacken, B
AU  - Benzacken B
AD  - Service d'Histologie, Embryologie, Cytogenetique et Biologie de la Reproduction, 
      Hopital Jean Verdier, Bondy, France.
FAU - Martin-Pont, B
AU  - Martin-Pont B
FAU - Bergere, M
AU  - Bergere M
FAU - Hugues, J N
AU  - Hugues JN
FAU - Wolf, J P
AU  - Wolf JP
FAU - Selva, J
AU  - Selva J
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - J Assist Reprod Genet
JT  - Journal of assisted reproduction and genetics
JID - 9206495
RN  - 0 (Fluorescent Dyes)
SB  - IM
MH  - Adult
MH  - Aneuploidy
MH  - Chromosome Aberrations/*pathology
MH  - Chromosome Disorders
MH  - *Chromosomes, Human, Pair 21
MH  - Female
MH  - Fertilization in Vitro
MH  - Fluorescent Dyes
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Infertility, Female/pathology
MH  - Infertility, Male/pathology
MH  - Male
MH  - *Maternal Age
MH  - Metaphase
MH  - Oocytes/*ultrastructure
MH  - Ovarian Follicle/physiology
MH  - Pregnancy
MH  - Sex Chromosome Aberrations/*pathology
MH  - *X Chromosome
PMC - PMC3454976
EDAT- 1998/04/21 00:00
MHDA- 1998/04/21 00:01
PMCR- 1999/03/01
CRDT- 1998/04/21 00:00
PHST- 1998/04/21 00:00 [pubmed]
PHST- 1998/04/21 00:01 [medline]
PHST- 1998/04/21 00:00 [entrez]
PHST- 1999/03/01 00:00 [pmc-release]
AID - 421880 [pii]
AID - 10.1023/a:1023056502731 [doi]
PST - ppublish
SO  - J Assist Reprod Genet. 1998 Mar;15(3):105-10. doi: 10.1023/a:1023056502731.