PMID- 9548167
OWN - NLM
STAT- MEDLINE
DCOM- 19980501
LR  - 20190707
IS  - 0015-0282 (Print)
IS  - 0015-0282 (Linking)
VI  - 69
IP  - 4
DP  - 1998 Apr
TI  - Simultaneous assessment of sperm chromatin condensation and morphology before and 
      after separation procedures: effect on the clinical outcome after in vitro 
      fertilization.
PG  - 740-7
AB  - OBJECTIVE: To look for correlations between acridine orange (AO) staining and 
      semen parameters before and after sperm separation procedures and to assess 
      whether the AO test predicts fertilization or pregnancy outcomes after standard 
      IVF and intracytoplasmic sperm injection. DESIGN: Prospective study that 
      simultaneously assesses sperm morphology and nuclear protein maturity on a 
      cell-by-cell basis before and after preparative procedures. SETTING: University 
      teaching hospital. PATIENT(S): Men (n = 140) undergoing diagnostic semen 
      analysis. MAIN OUTCOME MEASURE(S): Acridine orange fluorescence of sperm nuclei, 
      semen parameters, IVF outcome. RESULT(S): In unprocessed samples, 90% of sperm 
      with normal heads displayed green fluorescence (mature nuclear protein); 
      significantly lower percentages of green fluorescence were observed in sperm with 
      abnormal heads. The percentage of mature normal sperm in the specimen correlated 
      with motility. Sperm maturity after swim-up or Percoll gradient was significantly 
      improved for sperm with normal or abnormal heads. The percentage of mature normal 
      sperm correlated with motility after either Percoll or swim-up. Neither the 
      percentages of mature nuclei nor mature normal nuclei correlated with 
      fertilization or pregnancy outcome. CONCLUSION(S): Nuclear protein maturation 
      correlates with sperm motility and morphology. Because morphologically normal and 
      motile sperm are more mature, separation procedures should generate a population 
      of sperm with the highest fertilization capacity. Acridine orange staining, 
      however, did not predict fertilization efficiency or pregnancy outcome in IVF 
      cycles.
FAU - Angelopoulos, T
AU  - Angelopoulos T
AD  - Department of Obstetrics and Gynecology, New York University Medical Center, New 
      York 10016, USA.
FAU - Moshel, Y A
AU  - Moshel YA
FAU - Lu, L
AU  - Lu L
FAU - Macanas, E
AU  - Macanas E
FAU - Grifo, J A
AU  - Grifo JA
FAU - Krey, L C
AU  - Krey LC
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - Fertil Steril
JT  - Fertility and sterility
JID - 0372772
RN  - 0 (Chromatin)
RN  - 0 (Colloids)
RN  - 0 (Fluorescent Dyes)
RN  - 65455-52-9 (Percoll)
RN  - 7631-86-9 (Silicon Dioxide)
RN  - F30N4O6XVV (Acridine Orange)
RN  - FZ989GH94E (Povidone)
SB  - IM
MH  - Acridine Orange/chemistry
MH  - Adult
MH  - Centrifugation, Density Gradient
MH  - Chromatin/chemistry/*ultrastructure
MH  - Cohort Studies
MH  - Colloids
MH  - *Fertilization in Vitro
MH  - Fluorescent Dyes/chemistry
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Povidone/chemistry
MH  - Prospective Studies
MH  - Silicon Dioxide/chemistry
MH  - Sperm Motility/physiology
MH  - Spermatozoa/chemistry/*physiology/*ultrastructure
EDAT- 1998/04/21 00:00
MHDA- 1998/04/21 00:01
CRDT- 1998/04/21 00:00
PHST- 1998/04/21 00:00 [pubmed]
PHST- 1998/04/21 00:01 [medline]
PHST- 1998/04/21 00:00 [entrez]
AID - S0015-0282(98)00016-8 [pii]
AID - 10.1016/s0015-0282(98)00016-8 [doi]
PST - ppublish
SO  - Fertil Steril. 1998 Apr;69(4):740-7. doi: 10.1016/s0015-0282(98)00016-8.