PMID- 9548214
OWN - NLM
STAT- MEDLINE
DCOM- 19980519
LR  - 20190813
IS  - 0303-7207 (Print)
IS  - 0303-7207 (Linking)
VI  - 136
IP  - 2
DP  - 1998 Jan 15
TI  - Ligand-inducible retinoid X receptor-mediated protein: DNA interactions in the 
      retinoic acid receptor beta2 gene promoter in vivo.
PG  - 109-18
AB  - Retinoid X receptors (RXRs) are recently characterized transcription factors that 
      are members of the nuclear hormone receptor superfamily. However, it is not known 
      whether the endogenous RXR complex requires its ligand for access to its hormone 
      response element (HRE) of a target gene in vivo. Hence, dimethyl sulfate-based 
      genomic footprinting was carried out to examine occupancy of HREs in the retinoic 
      acid (RA) receptor beta2 (RARbeta2) gene promoter in the murine melanoma cell 
      line S91 cultured in the absence or presence of T3, all-trans-RA (atRA), or 
      CD2624, an RXR-selective retinoid. No footprint was observed at the RA-response 
      element (betaRARE) in the absence of ligands. However, a footprint was detected 
      at the betaRARE and other cis-acting elements after a 6 h incubation with CD2624 
      and atRA. Interestingly, only the betaRARE was footprinted after 60 min 
      incubation with CD2624. These results suggest that the endogenous RXR complex can 
      interact with an HRE of a target gene in the presence of ligand, and subsequently 
      may initiate additional interactions between DNA and other transcription factors.
FAU - Ikeda, M
AU  - Ikeda M
AD  - The Third Department of Internal Medicine, Yamanashi Medical University, Tamaho, 
      Japan.
FAU - Spanjaard, R A
AU  - Spanjaard RA
FAU - Noordhoek, E W
AU  - Noordhoek EW
FAU - Kawaguchi, A
AU  - Kawaguchi A
FAU - Onaya, T
AU  - Onaya T
FAU - Chin, W W
AU  - Chin WW
LA  - eng
GR  - DK 50300/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Ireland
TA  - Mol Cell Endocrinol
JT  - Molecular and cellular endocrinology
JID - 7500844
RN  - 0 (Benzoates)
RN  - 0 (CD 2624)
RN  - 0 (Naphthalenes)
RN  - 0 (Receptors, Retinoic Acid)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Retinoid X Receptors)
RN  - 0 (Transcription Factors)
RN  - 06LU7C9H1V (Triiodothyronine)
RN  - 5688UTC01R (Tretinoin)
RN  - 9007-49-2 (DNA)
RN  - EC 1.13.12.- (Luciferases)
SB  - IM
MH  - Animals
MH  - Benzoates/pharmacology
MH  - DNA/*metabolism
MH  - DNA Footprinting
MH  - Dimerization
MH  - Gene Expression Regulation/drug effects
MH  - Luciferases/genetics
MH  - Melanoma, Experimental/metabolism
MH  - Mice
MH  - Naphthalenes/pharmacology
MH  - *Promoter Regions, Genetic
MH  - Receptors, Retinoic Acid/*genetics/*physiology
MH  - Recombinant Fusion Proteins
MH  - Retinoid X Receptors
MH  - Transcription Factors/*metabolism/*physiology
MH  - Transfection
MH  - Tretinoin/pharmacology
MH  - Triiodothyronine/pharmacology
MH  - Tumor Cells, Cultured
EDAT- 1998/04/21 00:00
MHDA- 1998/04/21 00:01
CRDT- 1998/04/21 00:00
PHST- 1998/04/21 00:00 [pubmed]
PHST- 1998/04/21 00:01 [medline]
PHST- 1998/04/21 00:00 [entrez]
AID - S0303720797002177 [pii]
AID - 10.1016/s0303-7207(97)00217-7 [doi]
PST - ppublish
SO  - Mol Cell Endocrinol. 1998 Jan 15;136(2):109-18. doi: 
      10.1016/s0303-7207(97)00217-7.