PMID- 9548500
OWN - NLM
STAT- MEDLINE
DCOM- 19980420
LR  - 20131121
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 159
IP  - 11
DP  - 1997 Dec 1
TI  - Alteration of the cytokine phenotype in an experimental lung granuloma model by 
      inhibiting nitric oxide.
PG  - 5585-93
AB  - Pulmonary granulomatous inflammation modulated by IFN-gamma and IL-12 is also 
      associated with augmented inducible nitric oxide synthase (NOS II). To address 
      the role of increased nitric oxide synthesis in this model, mice received daily 
      i.p. injections of NG-nitro-L-arginine-methyl ester (L-NAME; 8 mg/kg) during both 
      the 2-wk immunization period with purified protein-derivative (PPD) and the 
      subsequent lung challenge with PPD-coated Sepharose beads. Other groups of 
      animals received saline, L-NAME or NG-nitro-D-arginine-methyl ester (D-NAME; 8 
      mg/kg) during the pulmonary embolization period and not the PPD sensitization 
      period. On day 4 post-PPD bead challenge, PCR analysis of the whole lung revealed 
      that NOS II expression appeared to be similar in both of the L-NAME treatment 
      protocols. L-NAME-treated mice in both dosing protocols had lung lesions that 
      were significantly larger than granuloma lesions measured in mice that received 
      saline or D-NAME. The enlarged lesions from L-NAME-treated mice contained 
      markedly greater numbers of neutrophils and eosinophils. Equivalent numbers of 
      PPD-activated dispersed cells from whole lungs of L-NAME-treated mice produced 
      significantly higher levels of IL-4 and IL-10 and smaller amounts of IL-12 and 
      IFN-gamma compared with similar lung cultures derived from control or 
      D-NAME-treated mice. Levels of C-C chemokines such as monocyte chemoattractant 
      protein-1 (MCP-1), C10, and macrophage inflammatory protein-1alpha (MIP-1alpha) 
      were also significantly elevated in lung cultures from L-NAME-treated mice 
      compared with controls. Thus, nitric oxide regulates the size and cellular 
      composition of the Th1-type lung granuloma, possibly through its effects on the 
      cytokine and chemokine profile associated with this lesion.
FAU - Hogaboam, C M
AU  - Hogaboam CM
AD  - Department of Pathology, University of Michigan Medical School, Ann Arbor 
      48109-0602, USA.
FAU - Chensue, S W
AU  - Chensue SW
FAU - Steinhauser, M L
AU  - Steinhauser ML
FAU - Huffnagle, G B
AU  - Huffnagle GB
FAU - Lukacs, N W
AU  - Lukacs NW
FAU - Strieter, R M
AU  - Strieter RM
FAU - Kunkel, S L
AU  - Kunkel SL
LA  - eng
GR  - 1P50HL56402/HL/NHLBI NIH HHS/United States
GR  - HL31963/HL/NHLBI NIH HHS/United States
GR  - HL35276/HL/NHLBI NIH HHS/United States
GR  - etc.
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Cytokines)
RN  - 0 (Tuberculin)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 130068-27-8 (Interleukin-10)
RN  - 187348-17-0 (Interleukin-12)
RN  - 207137-56-2 (Interleukin-4)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - 82115-62-6 (Interferon-gamma)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type II)
RN  - EC 1.14.13.39 (Nos2 protein, mouse)
RN  - V55S2QJN2X (NG-Nitroarginine Methyl Ester)
SB  - IM
MH  - Animals
MH  - Cytokines/*metabolism
MH  - Eosinophils/immunology
MH  - Female
MH  - Granuloma, Respiratory Tract/*immunology
MH  - Interferon-gamma/metabolism
MH  - Interleukin-10/metabolism
MH  - Interleukin-12/metabolism
MH  - Interleukin-4/metabolism
MH  - Lung/enzymology
MH  - Lung Diseases/*immunology
MH  - Mice
MH  - Mice, Inbred CBA
MH  - NG-Nitroarginine Methyl Ester/pharmacology
MH  - Neutrophils/immunology
MH  - Nitric Oxide/antagonists & inhibitors/biosynthesis/*physiology
MH  - Nitric Oxide Synthase/metabolism
MH  - Nitric Oxide Synthase Type II
MH  - *Th1 Cells
MH  - Tuberculin/pharmacology
MH  - Tumor Necrosis Factor-alpha/metabolism
EDAT- 1998/04/21 00:00
MHDA- 1998/04/21 00:01
CRDT- 1998/04/21 00:00
PHST- 1998/04/21 00:00 [pubmed]
PHST- 1998/04/21 00:01 [medline]
PHST- 1998/04/21 00:00 [entrez]
PST - ppublish
SO  - J Immunol. 1997 Dec 1;159(11):5585-93.