PMID- 9551602
OWN - NLM
STAT- MEDLINE
DCOM- 19980608
LR  - 20191102
IS  - 0196-4763 (Print)
IS  - 0196-4763 (Linking)
VI  - 31
IP  - 4
DP  - 1998 Apr 1
TI  - Detection of HIV-RNA-positive monocytes in peripheral blood of HIV-positive 
      patients by simultaneous flow cytometric analysis of intracellular HIV RNA and 
      cellular immunophenotype.
PG  - 265-74
AB  - Determinations of plasma HIV viral RNA copy numbers help to define the kinetics 
      of HIV-1 infection in vivo and to monitor antiretroviral therapy. However, 
      questions remain regarding the identity of various infected cell types 
      contributing to this free virus pool and to the in vivo lifecycle of HIV during 
      disease progression. Characterization of a novel fluorescence in situ 
      hybridization (FISH) assay employing a pool of labeled oligonucleotide probes 
      directed against HIV RNA was done followed by coupling of the FISH assay with 
      simultaneous surface immunophenotyping to address these questions. In vitro 
      characterizations of this assay using tumor necrosis factor-alpha stimulated and 
      unstimulated ACH-2 cells demonstrated the ability to detect < 5% HIV RNA positive 
      cells with a sensitivity of < 30 RNA copies per cell. Peripheral blood 
      mononuclear cells from 39 HIV-seropositive patients on no, single, combination, 
      or triple drug therapy and 8 HIV-seronegative patients were examined. The 
      majority of HIV-positive patients (24/39) harbored monocytes positive for HIV RNA 
      and a significantly higher fraction of patients with high plasma viral load 
      carried positive monocytes (13/16) than did patients in the low plasma viral load 
      group (11/23). These results demonstrate the effectiveness of a novel FISH assay 
      for identifying and monitoring HIV-infected cell populations in the peripheral 
      blood of HIV-positive patients. In addition, monocytes are a major source of 
      cellular HIV virus in the peripheral blood of HIV patients, even with progression 
      of disease.
FAU - Patterson, B K
AU  - Patterson BK
AD  - Department of Obstetrics and Gynecology, Northwestern University Medical School, 
      Department of Medicine, Chicago, Illinois 60611, USA. bpatters@nmh.org
FAU - Mosiman, V L
AU  - Mosiman VL
FAU - Cantarero, L
AU  - Cantarero L
FAU - Furtado, M
AU  - Furtado M
FAU - Bhattacharya, M
AU  - Bhattacharya M
FAU - Goolsby, C
AU  - Goolsby C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cytometry
JT  - Cytometry
JID - 8102328
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Cell Line
MH  - Flow Cytometry
MH  - HIV Infections/*virology
MH  - HIV-1/*isolation & purification
MH  - Humans
MH  - Immunophenotyping
MH  - In Situ Hybridization, Fluorescence/methods/statistics & numerical data
MH  - Kinetics
MH  - Monocytes/virology
MH  - Polymerase Chain Reaction
MH  - RNA, Viral/*blood
MH  - Sensitivity and Specificity
MH  - Viremia/virology
EDAT- 1998/04/29 06:28
MHDA- 2000/06/20 09:00
CRDT- 1998/04/29 06:28
PHST- 1998/04/29 06:28 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1998/04/29 06:28 [entrez]
AID - 10.1002/(SICI)1097-0320(19980401)31:4<265::AID-CYTO6>3.0.CO;2-I [pii]
AID - 10.1002/(sici)1097-0320(19980401)31:4<265::aid-cyto6>3.0.co;2-i [doi]
PST - ppublish
SO  - Cytometry. 1998 Apr 1;31(4):265-74. doi: 
      10.1002/(sici)1097-0320(19980401)31:4<265::aid-cyto6>3.0.co;2-i.