PMID- 9554281
OWN - NLM
STAT- MEDLINE
DCOM- 19980625
LR  - 20181201
IS  - 0301-5149 (Print)
IS  - 0301-5149 (Linking)
VI  - 92
DP  - 1998
TI  - Modulation of the immune response to tetanus toxoid by polylactide-polyglycolide 
      microspheres.
PG  - 259-67
AB  - The cellular and antibody responses in mice to tetanus toxoid (TT), alone or 
      adsorbed to aluminium hydroxide TT were compared to those obtained with TT 
      incorporated in polylactide-polyglycolide (PLGA) microspheres, a candidate single 
      dose vaccine. After subcutaneous injection, the serum IgM antibody to TT followed 
      similar overall patterns for all preparations although the highest levels were 
      achieved with the alum-adsorbed material. A similar pattern was observed with the 
      overall IgG antibody responses. The isotype distribution of antibodies was 
      broadly similar for all types of preparation although considerable qualitative 
      differences were observed. IgG1 antibodies predominated although IgG2a and IgG2b 
      made a substantial contribution, especially at three months in the case of the 
      two adjuvanted preparations. The IgG3 responses to each type of vaccine were very 
      low. Both alum and microsphere vaccines induced proliferative responses to TT in 
      splenic lymphocytes at three months after vaccination. Each type of vaccine was 
      much less effective in inducing proliferative responses in lymph node cells. 
      There was evidence of induction of IL2, IL4 and interferon-gamma genes by 
      microsphere vaccines in splenic but not lymph node cells. There were indications 
      that PLGA microspheres alone exerted a modulating effect on cellular responses 
      after immunization. These results suggest that TT encapsulated in microspheres 
      induces a pattern of cellular and antibody responses qualitatively similar to 
      those induced by conventional TT vaccines.
FAU - Walker, K B
AU  - Walker KB
AD  - National Institute for Biological Standards and Control, Hertfordshire, U.K.
FAU - Xing, D K
AU  - Xing DK
FAU - Sesardic, D
AU  - Sesardic D
FAU - Corbel, M J
AU  - Corbel MJ
LA  - eng
PT  - Journal Article
PL  - Switzerland
TA  - Dev Biol Stand
JT  - Developments in biological standardization
JID - 0427140
RN  - 0 (Immunoglobulin Isotypes)
RN  - 0 (Polymers)
RN  - 0 (Tetanus Toxoid)
RN  - 1SIA8062RS (Polylactic Acid-Polyglycolic Acid Copolymer)
RN  - 26009-03-0 (Polyglycolic Acid)
RN  - 33X04XA5AT (Lactic Acid)
SB  - IM
MH  - Animals
MH  - Female
MH  - Immunity, Cellular
MH  - Immunoenzyme Techniques
MH  - Immunoglobulin Isotypes/biosynthesis
MH  - *Lactic Acid
MH  - Mice
MH  - Microspheres
MH  - *Polyglycolic Acid
MH  - Polylactic Acid-Polyglycolic Acid Copolymer
MH  - *Polymers
MH  - Sheep
MH  - Tetanus Toxoid/*immunology
EDAT- 1998/04/29 00:00
MHDA- 1998/04/29 00:01
CRDT- 1998/04/29 00:00
PHST- 1998/04/29 00:00 [pubmed]
PHST- 1998/04/29 00:01 [medline]
PHST- 1998/04/29 00:00 [entrez]
PST - ppublish
SO  - Dev Biol Stand. 1998;92:259-67.