PMID- 9554849
OWN - NLM
STAT- MEDLINE
DCOM- 19980507
LR  - 20190619
IS  - 0036-8075 (Print)
IS  - 0036-8075 (Linking)
VI  - 280
IP  - 5363
DP  - 1998 Apr 24
TI  - Visualization of single RNA transcripts in situ.
PG  - 585-90
AB  - Fluorescence in situ hybridization (FISH) and digital imaging microscopy were 
      modified to allow detection of single RNA molecules. Oligodeoxynucleotide probes 
      were synthesized with five fluorochromes per molecule, and the light emitted by a 
      single probe was calibrated. Points of light in exhaustively deconvolved images 
      of hybridized cells gave fluorescent intensities and distances between probes 
      consistent with single messenger RNA molecules. Analysis of beta-actin 
      transcription sites after serum induction revealed synchronous and cyclical 
      transcription from single genes. The rates of transcription initiation and 
      termination and messenger RNA processing could be determined by positioning 
      probes along the transcription unit. This approach extends the power of FISH to 
      yield quantitative molecular information on a single cell.
FAU - Femino, A M
AU  - Femino AM
AD  - Department of Anatomy and Structural Biology and Cell Biology, Albert Einstein 
      College of Medicine, Bronx, NY 10461, USA.
FAU - Fay, F S
AU  - Fay FS
FAU - Fogarty, K
AU  - Fogarty K
FAU - Singer, R H
AU  - Singer RH
LA  - eng
GR  - GM 54887/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Science
JT  - Science (New York, N.Y.)
JID - 0404511
RN  - 0 (Actins)
RN  - 0 (Oligonucleotide Probes)
RN  - 0 (RNA, Messenger)
RN  - I223NX31W9 (Fluorescein-5-isothiocyanate)
SB  - IM
MH  - Actins/genetics
MH  - Animals
MH  - Cell Line
MH  - Fluorescein-5-isothiocyanate
MH  - *In Situ Hybridization, Fluorescence
MH  - Kinetics
MH  - Oligonucleotide Probes
MH  - RNA Processing, Post-Transcriptional
MH  - RNA, Messenger/*analysis/*genetics/metabolism
MH  - Rats
MH  - *Transcription, Genetic
EDAT- 1998/05/09 00:00
MHDA- 1998/05/09 00:01
CRDT- 1998/05/09 00:00
PHST- 1998/05/09 00:00 [pubmed]
PHST- 1998/05/09 00:01 [medline]
PHST- 1998/05/09 00:00 [entrez]
AID - 10.1126/science.280.5363.585 [doi]
PST - ppublish
SO  - Science. 1998 Apr 24;280(5363):585-90. doi: 10.1126/science.280.5363.585.