PMID- 9554946
OWN - NLM
STAT- MEDLINE
DCOM- 19980618
LR  - 20190831
IS  - 0165-3806 (Print)
IS  - 0165-3806 (Linking)
VI  - 106
IP  - 1-2
DP  - 1998 Mar 12
TI  - Hippocampal nitric oxide synthase in the fetal guinea pig: effects of chronic 
      prenatal ethanol exposure.
PG  - 39-46
AB  - The effects of chronic maternal administration of ethanol on nitric oxide 
      synthase (NOS) activity and the numbers of NOS containing neurons, and CA1 and 
      CA3 pyramidal neurons in the hippocampus of the near term fetal guinea pig at 
      gestational day (GD) 62 were investigated. Pregnant guinea pigs received oral 
      administration of 4 g ethanol/kg maternal body weight (n = 5), isocaloric 
      sucrose/pair feeding (n = 5) or water (n = 5), or no treatment (NT; n = 5) from 
      GD 2 to GD 61. NOS activity in the 25,000 x g supernatant of hippocampal 
      homogenate was determined using a radiometric assay. The numbers of NOS 
      containing neurons, and CA1 and CA3 pyramidal neurons were determined using NADPH 
      diaphorase histochemistry and cresyl violet staining, respectively. The chronic 
      ethanol regimen produced a maternal blood ethanol concentration of 193 +/- 13 
      mg/dl at 1 h after the second divided dose on GD 57. Chronic ethanol exposure 
      produced fetal body, brain, and hippocampal growth restriction and decreased 
      fetal hippocampal NOS activity compared with the isocaloric sucrose/pair feeding, 
      water, and NT experimental groups, but did not affect the number of NOS 
      containing and CA1 or CA3 pyramidal neurons. These data demonstrate that, in the 
      near term fetus, chronic maternal administration of ethanol suppresses 
      hippocampal NOS activity and consequent formation of NO, without loss of NOS 
      containing neurons and prior to loss of CA1 pyramidal neurons that occurs in the 
      adult.
FAU - Kimura, K A
AU  - Kimura KA
AD  - Department of Pharmacology and Toxicology, Faculty of Health Sciences, Queen's 
      University, Kingston, Ontario, Canada.
FAU - Brien, J F
AU  - Brien JF
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Brain Res Dev Brain Res
JT  - Brain research. Developmental brain research
JID - 8908639
RN  - 3K9958V90M (Ethanol)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase)
RN  - EC 1.6.99.1 (NADPH Dehydrogenase)
SB  - IM
MH  - Animals
MH  - Body Weight
MH  - Brain/embryology
MH  - Cell Count
MH  - Disease Models, Animal
MH  - Ethanol/*blood
MH  - Female
MH  - Fetal Alcohol Spectrum Disorders/*enzymology
MH  - Guinea Pigs
MH  - Hippocampus/cytology/embryology/*enzymology
MH  - *Maternal-Fetal Exchange
MH  - NADPH Dehydrogenase/analysis
MH  - Neurons/*enzymology
MH  - Nitric Oxide Synthase/*metabolism
MH  - Organ Size
MH  - Pregnancy
MH  - Pregnancy Outcome
MH  - Pyramidal Cells/enzymology
EDAT- 1998/04/29 00:00
MHDA- 1998/04/29 00:01
CRDT- 1998/04/29 00:00
PHST- 1998/04/29 00:00 [pubmed]
PHST- 1998/04/29 00:01 [medline]
PHST- 1998/04/29 00:00 [entrez]
AID - S0165-3806(97)00184-3 [pii]
AID - 10.1016/s0165-3806(97)00184-3 [doi]
PST - ppublish
SO  - Brain Res Dev Brain Res. 1998 Mar 12;106(1-2):39-46. doi: 
      10.1016/s0165-3806(97)00184-3.