PMID- 9554971
OWN - NLM
STAT- MEDLINE
DCOM- 19980723
LR  - 20190614
IS  - 0006-8993 (Print)
IS  - 0006-8993 (Linking)
VI  - 788
IP  - 1-2
DP  - 1998 Mar 30
TI  - Regulation of G protein-mediated adenylyl cyclase in striatum and cortex of 
      opiate-dependent and opiate withdrawing mice.
PG  - 104-10
AB  - Previous research has demonstrated that acute and chronic opiate treatment alters 
      receptor- and postreceptor-mediated adenylyl cyclase activity. This study 
      examined the regulation of G protein- and forskolin-mediated adenylyl cyclase 
      activity in mouse striatum and cortex after short- and long-term opiate exposure. 
      To directly measure adenylyl cyclase enzymatic activity, assays were done in the 
      presence of catalytic site activator forskolin. To measure G protein-mediated 
      adenylyl cyclase activity, assays were performed in the presence of 
      non-hydrolyzable guanosine 5'-triphosphate (GTP) analogue, 
      5'-guanylyl-imidodiphosphate. Short-term in vitro morphine exposure produced 
      reductions in forskolin-stimulated adenylyl cyclase activity in striatal and 
      cortical tissues. Long-term morphine treatment in mice was performed via 
      morphine- or placebo-pellet implantation for 72 h; this treatment has been shown 
      to produce opiate dependence and withdrawal. In both opiate-dependent and opiate 
      withdrawing mice (1 h post-naloxone induction), there were significant increases 
      in G protein-mediated adenylyl cyclase activity in the striatum (vs. controls). 
      In opiate-dependent mice, there was an decrease in G protein-mediated adenylyl 
      cyclase activity in cortex. In opiate-dependent mice, there were no changes in 
      forskolin-stimulated adenylyl cyclase in the striatum or cortex. Increases in 
      striatal G protein-mediated adenylyl cyclase could represent a compensatory 
      adaptation that opposes the persistent inhibition of adenylyl cyclase by chronic 
      opiate treatment contributing to the expression of opiate dependence and 
      withdrawal.
CI  - Copyright 1998 Elsevier Science B.V.
FAU - Kaplan, G B
AU  - Kaplan GB
AD  - Department of Psychiatry and Human Behavior, Veterans Affairs Medical Center and 
      Brown University School of Medicine, Providence, RI 02908, USA.
FAU - Sethi, R K
AU  - Sethi RK
FAU - McClelland, E G
AU  - McClelland EG
FAU - Leite-Morris, K A
AU  - Leite-Morris KA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - Netherlands
TA  - Brain Res
JT  - Brain research
JID - 0045503
RN  - 1F7A44V6OU (Colforsin)
RN  - 76I7G6D29C (Morphine)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
RN  - EC 4.6.1.1 (Adenylyl Cyclases)
SB  - IM
MH  - Adenylyl Cyclases/*metabolism
MH  - Analysis of Variance
MH  - Animals
MH  - Cerebral Cortex/drug effects
MH  - Colforsin/pharmacology
MH  - Corpus Striatum/drug effects
MH  - GTP-Binding Proteins/*physiology
MH  - Logistic Models
MH  - Male
MH  - Mice
MH  - Mice, Inbred Strains
MH  - Morphine/*adverse effects
MH  - Morphine Dependence/*enzymology
MH  - *Substance Withdrawal Syndrome
MH  - Telencephalon/*drug effects/enzymology
EDAT- 1998/05/21 00:00
MHDA- 1998/05/21 00:01
CRDT- 1998/05/21 00:00
PHST- 1998/05/21 00:00 [pubmed]
PHST- 1998/05/21 00:01 [medline]
PHST- 1998/05/21 00:00 [entrez]
AID - S0006-8993(97)01524-2 [pii]
AID - 10.1016/s0006-8993(97)01524-2 [doi]
PST - ppublish
SO  - Brain Res. 1998 Mar 30;788(1-2):104-10. doi: 10.1016/s0006-8993(97)01524-2.