PMID- 9557603
OWN - NLM
STAT- MEDLINE
DCOM- 19980504
LR  - 20190914
IS  - 0887-6924 (Print)
IS  - 0887-6924 (Linking)
VI  - 12
IP  - 4
DP  - 1998 Apr
TI  - Comparative genomic hybridization as part of a new diagnostic strategy in 
      childhood hyperdiploid acute lymphoblastic leukemia.
PG  - 474-81
AB  - The detailed definition of karyotype changes associated with hyperdiploid acute 
      lymphoblastic leukemia (ALL) is a precondition for their exploitation in minimal 
      residual disease studies with fluorescence in situ hybridization analysis (FISH). 
      In addition, certain karyotype patterns may have different prognostic 
      implications. We have therefore used comparative genomic hybridization (CGH) to 
      analyze the quantitative karyotype abnormalities in 14 cases of hyperdiploid ALL 
      and correlated the results with those obtained by flow cytometry and conventional 
      cytogenetic analyses. Despite an overall good agreement between the karyotypes 
      obtained by classical banding techniques and CGH, we came across at least one 
      karyotype discrepancy per case. Clarification of the discordant findings with 
      fluorescence in situ hybridization (FISH) showed that all stem lines had been 
      correctly defined by CGH. In eight cases, however, cytogenetic analyses revealed 
      structural abnormalities that were undetectable by CGH. The other discrepancies 
      were mainly due to a cytogenetic misinterpretation of similar sized and shaped 
      chromosomes. Based on these findings we present a new diagnostic strategy for 
      childhood ALL that includes flow cytometry and classical cytogenetics as well as 
      CGH for the analysis of aneuploid cases and FISH to resolve the unavoidable 
      discrepancies.
FAU - Haas, O
AU  - Haas O
AD  - Children's Cancer Research Institute, St Anna Children's Hospital, Vienna, 
      Austria.
FAU - Henn, T
AU  - Henn T
FAU - Romanakis, K
AU  - Romanakis K
FAU - du Manoir, S
AU  - du Manoir S
FAU - Lengauer, C
AU  - Lengauer C
LA  - eng
PT  - Clinical Trial
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Leukemia
JT  - Leukemia
JID - 8704895
RN  - 0 (DNA, Neoplasm)
SB  - IM
MH  - Adolescent
MH  - Child
MH  - Child, Preschool
MH  - Chromosome Banding
MH  - DNA, Neoplasm/analysis/genetics
MH  - *Diploidy
MH  - Female
MH  - Flow Cytometry
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Karyotyping
MH  - Male
MH  - Nucleic Acid Hybridization/*methods
MH  - Precursor Cell Lymphoblastic Leukemia-Lymphoma/*diagnosis/*genetics
EDAT- 1998/04/29 00:00
MHDA- 1998/04/29 00:01
CRDT- 1998/04/29 00:00
PHST- 1998/04/29 00:00 [pubmed]
PHST- 1998/04/29 00:01 [medline]
PHST- 1998/04/29 00:00 [entrez]
AID - 10.1038/sj.leu.2400943 [doi]
PST - ppublish
SO  - Leukemia. 1998 Apr;12(4):474-81. doi: 10.1038/sj.leu.2400943.