PMID- 9557752
OWN - NLM
STAT- MEDLINE
DCOM- 19980520
LR  - 20200724
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 72
IP  - 5
DP  - 1998 May
TI  - Identification of a putative receptor for porcine reproductive and respiratory 
      syndrome virus on porcine alveolar macrophages.
PG  - 4520-3
AB  - To identify the receptor which may determine the macrophage tropism of porcine 
      reproductive and respiratory syndrome virus (PRRSV), monoclonal antibodies (MAbs) 
      against porcine alveolar macrophages (PAM) were produced. Two MAbs (41D3 and 
      41D5) which completely blocked PRRSV infection of PAM were further characterized. 
      It was found that they reduce the attachment of PRRSV to PAM and 
      immunoprecipitate a 210-kDa membrane protein from PAM. This protein was detected 
      on the cell membranes of PAM but not of PRRSV-nonpermissive cells. A 
      colocalization was found between the reactive sites of MAb 41D3 and PRRSV on PAM 
      membranes. All PRRSV-infected cells in tissues of experimentally infected pigs 
      reacted with MAb 41D3. Taken together, all these data suggest that the identified 
      210-kDa membrane protein is a putative receptor for PRRSV on porcine macrophages.
FAU - Duan, X
AU  - Duan X
AD  - Laboratory of Veterinary Virology, Faculty of Veterinary Medicine, University of 
      Ghent, Merelbeke, Belgium.
FAU - Nauwynck, H J
AU  - Nauwynck HJ
FAU - Favoreel, H W
AU  - Favoreel HW
FAU - Pensaert, M B
AU  - Pensaert MB
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (Receptors, Virus)
SB  - IM
MH  - Animals
MH  - Macrophages, Alveolar/*metabolism
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Porcine respiratory and reproductive syndrome virus/*metabolism
MH  - Receptors, Virus/immunology/*metabolism
MH  - Swine
PMC - PMC109698
EDAT- 1998/04/29 00:00
MHDA- 1998/04/29 00:01
PMCR- 1998/05/01
CRDT- 1998/04/29 00:00
PHST- 1998/04/29 00:00 [pubmed]
PHST- 1998/04/29 00:01 [medline]
PHST- 1998/04/29 00:00 [entrez]
PHST- 1998/05/01 00:00 [pmc-release]
AID - 1800 [pii]
AID - 10.1128/JVI.72.5.4520-4523.1998 [doi]
PST - ppublish
SO  - J Virol. 1998 May;72(5):4520-3. doi: 10.1128/JVI.72.5.4520-4523.1998.