PMID- 9558079
OWN - NLM
STAT- MEDLINE
DCOM- 19980504
LR  - 20171116
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 160
IP  - 8
DP  - 1998 Apr 15
TI  - Fas/Fas ligand signaling during gestational T cell development.
PG  - 3766-75
AB  - Most thymocytes express high levels of Fas Ag (Apo-1/CD95); however, the role of 
      Fas/Fas ligand-mediated apoptosis in thymocyte development remains unclear. 
      During gestational development of thymocytes in C57BL/6(B6) +/+ mice, the highest 
      levels of Fas ligand mRNA and Fas ligand protein expression were detected at 
      gestational day (GD) 15, and there was a ninefold decrease in Fas ligand mRNA 
      expression between GD 15 and 17 accompanied by a sixfold increase in Fas mRNA. 
      Apoptotic thymocytes were first detected in the medulla at GD 15, and increasing 
      numbers of cortical clusters and scattered, single apoptotic cells were present 
      on GD 16 and 17. Thus, early apoptosis correlated with high expression of Fas 
      ligand. High levels of Fas ligand mRNA were maintained throughout gestational 
      development in thymocytes of Fas-deficient B6-lpr/lpr mice, but cortical clusters 
      and scattered apoptotic cells were decreased relative to B6 +/+ mice before GD 
      17. Kinetic analysis of fetal thymic organ cultures treated with anti-Fas Ab 
      demonstrated that thymocytes become sensitive to Fas-mediated apoptosis during 
      the transition from the CD4-CD8- to the CD4+CD8+ phenotype. More mature CD4+CD8+ 
      thymocytes and CD4+ and CD8+ thymocytes became resistant to Fas-mediated 
      apoptosis after GD 17, despite high expression of Fas. However, low avidity 
      engagement of the TCR on Fas-sensitive CD4+CD8+ thymocytes before GD 17 induced 
      resistance to Fas-mediated apoptosis. The present results indicate that Fas plays 
      a critical role in mediating apoptosis during early gestational thymocyte 
      development and that thymocytes that receive a survival signal through TCR/CD3 
      become resistant to Fas-mediated apoptosis.
FAU - Fleck, M
AU  - Fleck M
AD  - The University of Regensburg, Department of Medicine I, Germany.
FAU - Zhou, T
AU  - Zhou T
FAU - Tatsuta, T
AU  - Tatsuta T
FAU - Yang, P
AU  - Yang P
FAU - Wang, Z
AU  - Wang Z
FAU - Mountz, J D
AU  - Mountz JD
LA  - eng
GR  - P01-AR03555/AR/NIAMS NIH HHS/United States
GR  - R01-AR42567/AR/NIAMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (DNA Primers)
RN  - 0 (Fas Ligand Protein)
RN  - 0 (Fasl protein, mouse)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (fas Receptor)
SB  - IM
MH  - Animals
MH  - Apoptosis
MH  - Base Sequence
MH  - Cell Differentiation
MH  - DNA Primers/genetics
MH  - Embryonic and Fetal Development/genetics/immunology
MH  - Fas Ligand Protein
MH  - Female
MH  - Fetus/*cytology/*immunology/metabolism
MH  - Gene Expression Regulation, Developmental
MH  - Gestational Age
MH  - Male
MH  - Membrane Glycoproteins/genetics/*metabolism
MH  - Mice
MH  - Mice, Inbred MRL lpr
MH  - Organ Culture Techniques
MH  - Pregnancy
MH  - RNA, Messenger/genetics/metabolism
MH  - Signal Transduction
MH  - T-Lymphocytes/*cytology/*immunology/metabolism
MH  - fas Receptor/genetics/*metabolism
EDAT- 1998/04/29 00:00
MHDA- 1998/04/29 00:01
CRDT- 1998/04/29 00:00
PHST- 1998/04/29 00:00 [pubmed]
PHST- 1998/04/29 00:01 [medline]
PHST- 1998/04/29 00:00 [entrez]
PST - ppublish
SO  - J Immunol. 1998 Apr 15;160(8):3766-75.