PMID- 9558337
OWN - NLM
STAT- MEDLINE
DCOM- 19980520
LR  - 20061115
IS  - 0006-2960 (Print)
IS  - 0006-2960 (Linking)
VI  - 37
IP  - 17
DP  - 1998 Apr 28
TI  - Hepatocyte growth factor/scatter factor has distinct classes of binding site in 
      heparan sulfate from mammary cells.
PG  - 6003-8
AB  - Hepatocyte growth factor/scatter factor (HGF/SF) is a heparan sulfate 
      (HS)-binding growth factor and morphogen for mammary epithelial cells that is 
      produced by mammary stromal fibroblasts. HS chains, purified as peptidoglycans 
      from a panel of cell lines representative of the ductal epithelial cell (Huma 
      123), the myoepithelial cell (Huma 109), the stromal fibroblast (Rama 27), and 
      malignant mammary epithelial cells (MCF-7 and ZR-75), were used in a 
      biosensor-based assay to identify the classes of HGF/SF-binding sites in the 
      polysaccharide chains. At least three distinct binding sites were identified. One 
      site exhibits fast association and fast dissociation kinetics [kass (1.4-7.7) x 
      10(6) M-1 s-1; kdiss 0. 0032-0.0096 s-1] and is present on the HS from benign 
      Huma 123 epithelial cells, Huma 109 myoepithelial-like cells, and ZR-75 malignant 
      cells. The second binding site, found on HS from the malignant MCF-7 cells, has 
      slower HGF/SF-binding kinetics (kass 0.20 x 10(6) M-1 s-1; kdiss 0.00055 s-1). 
      The third binding site possesses fast association and slow dissociation kinetics 
      (kass 1.1 x 10(6) M-1 s-1; kdiss 0.00020 s-1) and was found on the HS isolated 
      from the culture medium of the Huma 123 benign epithelial cells. The first and 
      second binding sites have a similar Kd, 1-3 nM, while the third binding site has 
      a considerably higher affinity for HGF/SF (Kd 200 pM). The three binding sites 
      seem to be mutually exclusive, since each sample of HS possessed just one of the 
      sites.
FAU - Rahmoune, H
AU  - Rahmoune H
AD  - School of Biological Sciences, Life Sciences Building, University of Liverpool, 
      U.K.
FAU - Rudland, P S
AU  - Rudland PS
FAU - Gallagher, J T
AU  - Gallagher JT
FAU - Fernig, D G
AU  - Fernig DG
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochemistry
JT  - Biochemistry
JID - 0370623
RN  - 0 (Heparan Sulfate Proteoglycans)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (glycosaminoglycan receptor)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
SB  - IM
MH  - Animals
MH  - Binding Sites
MH  - Biosensing Techniques
MH  - Breast Neoplasms
MH  - Cell Line
MH  - Epithelial Cells/metabolism
MH  - Fibroblasts
MH  - Heparan Sulfate Proteoglycans/isolation & purification/*metabolism
MH  - Hepatocyte Growth Factor/*metabolism
MH  - Humans
MH  - Kinetics
MH  - Mammary Glands, Animal/cytology
MH  - Rats
MH  - Receptors, Cell Surface/analysis
MH  - Tumor Cells, Cultured
EDAT- 1998/05/23 00:00
MHDA- 1998/05/23 00:01
CRDT- 1998/05/23 00:00
PHST- 1998/05/23 00:00 [pubmed]
PHST- 1998/05/23 00:01 [medline]
PHST- 1998/05/23 00:00 [entrez]
AID - bi972468t [pii]
AID - 10.1021/bi972468t [doi]
PST - ppublish
SO  - Biochemistry. 1998 Apr 28;37(17):6003-8. doi: 10.1021/bi972468t.