PMID- 9560011
OWN - NLM
STAT- MEDLINE
DCOM- 19980612
LR  - 20211203
IS  - 0001-6322 (Print)
IS  - 0001-6322 (Linking)
VI  - 95
IP  - 4
DP  - 1998 Apr
TI  - Immunohistochemical examination of c-Met protein expression in astrocytic tumors.
PG  - 345-51
AB  - Hepatocyte growth factor/scatter factor (HGF/SF), which has various physiological 
      functions, and its receptor c-Met, the human c-met proto-oncogene product, are 
      thought to be determinant in the pathological processes of various malignancies. 
      To investigate the possible role of HGF/SF in the progression of development of 
      astrocytic tumors, we examined the expression of c-Met in these tumors. 
      Immunohistochemistry using the streptavidin-biotin peroxidase complex method and 
      immunofluorescence double staining with anti-c-Met polyclonal and anti-glial 
      fibrillary acidic protein monoclonal antibodies were performed. Positive c-Met 
      expression was detected in 31 of the 42 astrocytic tumors and some of the control 
      cases analyzed. c-Met-positive cells showed morphological characteristics of 
      astrocytes. Especially in the cases of high-grade tumors, c-Met positivity was 
      abundant in cells in both vascular-rich and peripheral regions of the tumors but 
      not in the cells with distinctly malignant features. Immunofluorescence double 
      staining revealed that the c-Met-positive cells were in part of astrocytic 
      origin. We suggest that c-Met-positive cells are affected by some factors in the 
      lesions where the pathological processes are in a state of development. Our 
      studies indicated that c-Met expression might take part in glioma invasion but 
      not in the development of malignancy.
FAU - Hirose, Y
AU  - Hirose Y
AD  - Department of Neurosurgery, Ashikaga Red Cross Hospital, Ashikaga-city, Tochigi, 
      Japan.
FAU - Kojima, M
AU  - Kojima M
FAU - Sagoh, M
AU  - Sagoh M
FAU - Murakami, H
AU  - Murakami H
FAU - Yoshida, K
AU  - Yoshida K
FAU - Shimazaki, K
AU  - Shimazaki K
FAU - Kawase, T
AU  - Kawase T
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - Acta Neuropathol
JT  - Acta neuropathologica
JID - 0412041
RN  - 0 (Glial Fibrillary Acidic Protein)
RN  - 0 (MAS1 protein, human)
RN  - 0 (Proto-Oncogene Mas)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
RN  - EC 2.7.10.1 (Proto-Oncogene Proteins c-met)
SB  - IM
MH  - Astrocytes/*metabolism
MH  - Astrocytoma/metabolism/pathology
MH  - Blotting, Northern
MH  - Brain Neoplasms/*metabolism/pathology
MH  - Fluorescent Antibody Technique, Direct
MH  - Glial Fibrillary Acidic Protein/metabolism
MH  - Glioma/*metabolism/pathology
MH  - Hepatocyte Growth Factor/physiology
MH  - Humans
MH  - Immunohistochemistry
MH  - Proto-Oncogene Mas
MH  - Proto-Oncogene Proteins c-met/*biosynthesis
EDAT- 1998/04/29 00:00
MHDA- 1998/04/29 00:01
CRDT- 1998/04/29 00:00
PHST- 1998/04/29 00:00 [pubmed]
PHST- 1998/04/29 00:01 [medline]
PHST- 1998/04/29 00:00 [entrez]
AID - 10.1007/s004010050809 [doi]
PST - ppublish
SO  - Acta Neuropathol. 1998 Apr;95(4):345-51. doi: 10.1007/s004010050809.