PMID- 9562186
OWN - NLM
STAT- MEDLINE
DCOM- 19980608
LR  - 20061115
IS  - 0894-1491 (Print)
IS  - 0894-1491 (Linking)
VI  - 23
IP  - 1
DP  - 1998 May
TI  - Partial peripheral nerve injury leads to activation of astroglia and microglia 
      which parallels the development of allodynic behavior.
PG  - 75-83
AB  - Three different cell markers were studied in rats to note changes in the 
      immunoreactivity (IR) in the lumbar spinal cord 1 to 84 days following partial 
      sciatic nerve ligation (PSNL). Alteration in average IR were studied for 
      complement receptor C3bi (OX42; microglia), major histocompatibility complex II 
      (OX6; microglia), and glia fibrillary acidic protein (GFAP; astroglia). 
      Thirty-four female rats underwent ligation of approximately 1/2 of the sciatic 
      nerve (PSNL). This injury resulted in the development of mechanical allodynia, 
      which was quantitated by the measurement of foot withdrawal threshold to the 
      application of Von Frey filaments. Ipsilateral increase in IR of OX42 and GFAP 
      was observed to occur within 2 days, maximized by day 14, and did not return to 
      contralateral spinal gray matter IR levels by day 84 (time period of study). 
      Increases in OX42 IR and GFAP IR were observed within the spinal segments 
      innervated by the sciatic nerve. GFAP IR was not expressed in all lumbar 
      segments. OX42 staining with the upper portion of the dorsal horn was found to 
      localize within the areas of deafferentation demonstrated by loss of thiamine 
      monophosphatase activity within the substantia gelatinosa. OX6 IR was only seen 
      in a few cells within the ipsilateral gray matter, indicating that microglia did 
      not become phagocytic. Both GFAP IR and OX42 IR were found to linearly correlate 
      with allodynic behavior with OX42 IR being more statistically significant. 
      Correlation of OX42 IR in only the upper portion of the dorsal horn (not 
      including the neck) resulted in an even a greater level of significance. These 
      findings demonstrate that microglia and astroglia are activated following PSNL 
      and that their increase in IR correlates with the development of allodynic 
      behavior.
FAU - Coyle, D E
AU  - Coyle DE
AD  - Department of Anesthesia, University of Cincinnati, Ohio 45367-0531, USA.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Glia
JT  - Glia
JID - 8806785
RN  - 0 (Glial Fibrillary Acidic Protein)
RN  - 0 (Histocompatibility Antigens Class II)
RN  - 0 (Receptors, Leukocyte-Adhesion)
SB  - IM
MH  - Animals
MH  - Astrocytes/*physiology
MH  - Female
MH  - Glial Fibrillary Acidic Protein/biosynthesis
MH  - Hindlimb
MH  - Histocompatibility Antigens Class II/biosynthesis
MH  - Microglia/*physiology
MH  - Pain/*physiopathology
MH  - Physical Stimulation
MH  - Rats
MH  - Receptors, Leukocyte-Adhesion/biosynthesis
MH  - Sciatic Nerve/*injuries/*physiology
MH  - Spinal Cord/*physiology
MH  - Time Factors
MH  - Touch
EDAT- 1998/04/30 02:03
MHDA- 2000/06/20 09:00
CRDT- 1998/04/30 02:03
PHST- 1998/04/30 02:03 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1998/04/30 02:03 [entrez]
AID - 10.1002/(SICI)1098-1136(199805)23:1<75::AID-GLIA7>3.0.CO;2-3 [pii]
PST - ppublish
SO  - Glia. 1998 May;23(1):75-83.