PMID- 9562854
OWN - NLM
STAT- MEDLINE
DCOM- 19980430
LR  - 20191102
IS  - 0928-0197 (Print)
IS  - 0928-0197 (Linking)
VI  - 9
IP  - 1
DP  - 1998 Jan
TI  - Molecular detection and isolation of human T-cell lymphotropic virus type I 
      (HTLV-I) from patients with HAM/TSP in Sao Paulo, Brazil.
PG  - 17-23
AB  - BACKGROUND: Infection with HTLV-I is etiologically linked with HTLV-I-associated 
      myelopathy/tropical spastic paraparesis (HAM/TSP). However some patients with 
      chronic progressive paraparesis resembling HAM/TSP have been shown to be infected 
      with HTLV-II. OBJECTIVE: To clarify the role of each of these human retroviruses 
      in the etiology of HAM/TSP in Sao Paulo, Brazil. STUDY DESIGN: A detailed 
      serological and molecular analysis of HTLV-I/II infection was performed in a 
      cohort of 19 patients with HAM/TSP attending a neurological clinic. RESULTS: 
      Plasma samples analyzed for anti-HTLV-I/II antibodies using a Western blot assay, 
      comprising HTLV-I (rgp46I)- and HTLV-II (rgp46II)-specific recombinant env 
      epitopes, demonstrated reactivity to rgp46I and hence were typed as seropositive 
      for HTLV-I. Presence of HTLV genomic sequences in peripheral blood mononuclear 
      cells (PBMC) was sought after by PCR using consensus primers SK 110 and SK 111 
      for the pol region of HTLV proviral DNA followed by hybridization with 
      type-specific probes--SK 112 (HTLV-I) and SK 188 (HTLV-II). Southern blots from 
      all individuals hybridized with SK 112 but not with SK 188, further confirming 
      HTLV-I infection. Cocultivation of PBMC from eight of these patients with 
      activated lymphocytes from normal individuals resulted in active viral 
      production, detected as presence of soluble p24gag antigen in culture 
      supernatants. Investigation of risk factors for HTLV-I infection in these 
      individuals revealed that five out of 19 patients studied (26.3%) had received 
      blood transfusions previous to disease onset.
FAU - Segurado, A A
AU  - Segurado AA
AD  - Department of Infectious Diseases, School of Medicine, University of Sao Paulo, 
      Brazil. segurado@usp.br
FAU - Domingues, R B
AU  - Domingues RB
FAU - Muniz, M R
AU  - Muniz MR
FAU - Fink, M C
AU  - Fink MC
FAU - Marchiori, P E
AU  - Marchiori PE
FAU - Scaff, M
AU  - Scaff M
FAU - Lal, R B
AU  - Lal RB
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Clin Diagn Virol
JT  - Clinical and diagnostic virology
JID - 9309653
RN  - 0 (Antigens, Viral)
RN  - 0 (Gene Products, gag)
RN  - 0 (Viral Envelope Proteins)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Antigens, Viral/immunology
MH  - Blotting, Western
MH  - Brazil
MH  - Female
MH  - Gene Products, gag/immunology
MH  - Human T-lymphotropic virus 1/genetics/immunology/*isolation & purification
MH  - Humans
MH  - Leukocytes, Mononuclear/virology
MH  - Male
MH  - Middle Aged
MH  - Paraparesis, Tropical Spastic/*virology
MH  - Sequence Analysis, DNA
MH  - Viral Envelope Proteins/immunology
EDAT- 1998/05/01 00:00
MHDA- 1998/05/01 00:01
CRDT- 1998/05/01 00:00
PHST- 1998/05/01 00:00 [pubmed]
PHST- 1998/05/01 00:01 [medline]
PHST- 1998/05/01 00:00 [entrez]
AID - S0928-0197(97)10015-0 [pii]
AID - 10.1016/s0928-0197(97)10015-0 [doi]
PST - ppublish
SO  - Clin Diagn Virol. 1998 Jan;9(1):17-23. doi: 10.1016/s0928-0197(97)10015-0.