PMID- 9568729
OWN - NLM
STAT- MEDLINE
DCOM- 19980619
LR  - 20131121
IS  - 1079-9907 (Print)
IS  - 1079-9907 (Linking)
VI  - 18
IP  - 4
DP  - 1998 Apr
TI  - IFN-gamma primes macrophage responses to bacterial DNA.
PG  - 263-71
AB  - Macrophages recognize and are activated by unmethylated CpG motifs in bacterial 
      DNA. Here we demonstrate that production of nitric oxide (NO) from murine RAW 264 
      macrophages and bone marrow-derived macrophages (BMM) in response to bacterial 
      DNA is absolutely dependent on interferon-gamma (IFN-gamma) priming. Similarly, 
      arginine uptake and expression of the inducible nitric oxide synthase (iNOS) gene 
      in response to bacterial DNA in BMM occurred only after IFN-gamma priming. In 
      contrast, mRNA for the cationic amino acid transporter, CAT2, was induced by 
      plasmid DNA alone, and priming with IFN-gamma had no effect on this response. 
      Tumor necrosis factor-alpha (TNF-alpha) release from RAW 264 and BMM in response 
      to bacterial DNA was augmented by IFN-gamma pretreatment. In a stably transfected 
      HIV-1 long terminal repeat (LTR) luciferase RAW 264 cell line, IFN-gamma and 
      bacterial DNA synergized in activation of the HIV-1 LTR. Bacterial DNA has been 
      shown to induce IFN-gamma production in vivo as an indirect consequence of 
      interleukin-12 (IL-12) and TNF-alpha production from macrophages. The results 
      herein suggest the existence of a self-amplifying loop that may have implications 
      for therapeutic applications of bacterial DNA.
FAU - Sweet, M J
AU  - Sweet MJ
AD  - Centre for Molecular and Cellular Biology, Department of Microbiology, University 
      of Queensland, Brisbane, Australia.
FAU - Stacey, K J
AU  - Stacey KJ
FAU - Kakuda, D K
AU  - Kakuda DK
FAU - Markovich, D
AU  - Markovich D
FAU - Hume, D A
AU  - Hume DA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Interferon Cytokine Res
JT  - Journal of interferon & cytokine research : the official journal of the 
      International Society for Interferon and Cytokine Research
JID - 9507088
RN  - 0 (DNA, Bacterial)
RN  - 0 (RNA, Messenger)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - 82115-62-6 (Interferon-gamma)
RN  - 94ZLA3W45F (Arginine)
RN  - EC 1.11.1.6 (Catalase)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type II)
RN  - EC 1.14.13.39 (Nos2 protein, mouse)
SB  - IM
MH  - Animals
MH  - Arginine/metabolism
MH  - Catalase/genetics
MH  - Cell Line
MH  - DNA, Bacterial/*genetics
MH  - HIV-1/genetics
MH  - Interferon-gamma/*pharmacology
MH  - Macrophages/*drug effects
MH  - Mice
MH  - Mice, Inbred Strains
MH  - Nitric Oxide/metabolism
MH  - Nitric Oxide Synthase/*genetics
MH  - Nitric Oxide Synthase Type II
MH  - RNA, Messenger/biosynthesis
MH  - Repetitive Sequences, Nucleic Acid
EDAT- 1998/05/06 00:00
MHDA- 1998/05/06 00:01
CRDT- 1998/05/06 00:00
PHST- 1998/05/06 00:00 [pubmed]
PHST- 1998/05/06 00:01 [medline]
PHST- 1998/05/06 00:00 [entrez]
AID - 10.1089/jir.1998.18.263 [doi]
PST - ppublish
SO  - J Interferon Cytokine Res. 1998 Apr;18(4):263-71. doi: 10.1089/jir.1998.18.263.