PMID- 9569039
OWN - NLM
STAT- MEDLINE
DCOM- 19980511
LR  - 20190515
IS  - 0007-0920 (Print)
IS  - 1532-1827 (Electronic)
IS  - 0007-0920 (Linking)
VI  - 77
IP  - 7
DP  - 1998 Apr
TI  - Expression of HGF/SF in mesothelioma cell lines and its effects on cell motility, 
      proliferation and morphology.
PG  - 1052-9
AB  - The expression of hepatocyte growth factor/scatter factor (HGF/SF) was studied in 
      12 mesothelioma cell lines characterized by either an epithelioid or a 
      fibroblast-like phenotype. Conditioned media from these lines were analysed by 
      bioassay and ELISA, and HGF/SF was detected in three cell lines, all with a 
      fibroblast-like or mixed morphology. None of eight epithelioid cell lines 
      expressed the factor. Thus, for these cell lines, the ability to secrete HGF/SF 
      correlated with the cell phenotype. Following on from these observations, two 
      cell lines, BR and BT, with a fibroblast-like and an epithelioid phenotype, 
      respectively, were further investigated. Both cell lines expressed the Met 
      receptor but only BR secreted HGF/SF. Both cell lines responded to exogenous 
      HGF/SF treatment by a change of morphology but in different ways: BR became more 
      elongated and bipolar, while BT formed more spread-out cell colonies. HGF/SF 
      acted as a paracrine effector on the epithelioid BT cells and stimulated both 
      cell-spreading and proliferation. Interestingly, BT cells spread but did not 
      scatter in response to exogenous HGF/SF. In contrast BR cells showed only some 
      stimulation of cell motility with HGF/SF and no increase in cell proliferation 
      was observed. Because HGF/SF was previously found in the pleural effusion fluids 
      of patients with malignant mesothelioma and in paraffin-embedded tumour tissues, 
      it is concluded that HGF/SF may well stimulate the growth and spread of malignant 
      mesothelioma in vivo by paracrine and/or autocrine mechanisms.
FAU - Harvey, P
AU  - Harvey P
AD  - School of Biology, University of East Anglia, Norwich, UK.
FAU - Warn, A
AU  - Warn A
FAU - Dobbin, S
AU  - Dobbin S
FAU - Arakaki, N
AU  - Arakaki N
FAU - Daikuhara, Y
AU  - Daikuhara Y
FAU - Jaurand, M C
AU  - Jaurand MC
FAU - Warn, R M
AU  - Warn RM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Br J Cancer
JT  - British journal of cancer
JID - 0370635
RN  - 0 (Neoplasm Proteins)
RN  - 42HK56048U (Tyrosine)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
RN  - EC 2.7.10.1 (Proto-Oncogene Proteins c-met)
SB  - IM
MH  - Cell Division
MH  - Cell Movement
MH  - Hepatocyte Growth Factor/genetics/*metabolism
MH  - Humans
MH  - Mesothelioma/genetics/*metabolism/*pathology
MH  - Neoplasm Proteins/genetics/*metabolism
MH  - Phosphorylation
MH  - Proto-Oncogene Proteins c-met/*metabolism
MH  - Tumor Cells, Cultured
MH  - Tyrosine/metabolism
PMC - PMC2150119
EDAT- 1998/05/06 07:06
MHDA- 2000/03/18 09:00
CRDT- 1998/05/06 07:06
PHST- 1998/05/06 07:06 [pubmed]
PHST- 2000/03/18 09:00 [medline]
PHST- 1998/05/06 07:06 [entrez]
AID - 10.1038/bjc.1998.176 [doi]
PST - ppublish
SO  - Br J Cancer. 1998 Apr;77(7):1052-9. doi: 10.1038/bjc.1998.176.