PMID- 9570568
OWN - NLM
STAT- MEDLINE
DCOM- 19980514
LR  - 20081121
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 160
IP  - 3
DP  - 1998 Feb 1
TI  - Streptococcal cell wall-induced arthritis: requirements for IL-4, IL-10, 
      IFN-gamma, and monocyte chemoattractant protein-1.
PG  - 1466-71
AB  - Intra-articular injection of streptococcal cell wall Ag followed by i.v. 
      challenge ("reactivation") results in a destructive lymphocyte-dependent 
      monoarticular arthritis. To further define the role of immune mechanisms in the 
      model, Abs to Th1 and Th2-related cytokines were evaluated. Treatment of rats 
      with antibodies to IL-4 reduced swelling, while treatment with anti-IL-10 or 
      anti-IFN-gamma either had no effect or slightly enhanced the inflammatory 
      response. These results suggest that Th-2 immune mechanisms may be, at least in 
      part, operative in the model. To more precisely define the role of IL-4, the 
      effects of anti-IL-4 on monocyte chemoattractant protein-1 (MCP-1) expression 
      were evaluated. Initial studies demonstrated that mRNA (as determined by in situ 
      hybridization) and protein (as determined by immunofluorescence) for MCP-1 were 
      detectable in inflamed synovial tissue in a time-dependent manner. Anti-IL-4 
      treatment significantly reduced the expression of mRNA for MCP-1 24 and 72 h 
      after reactivation. In addition, anti-MCP-1 inhibited swelling and reduced influx 
      of (111)In-labeled T cells. These data suggest that the reactivation model of 
      streptococcal cell wall Ag-induced arthritis is Th-2 dependent, and that an 
      inter-relationship exists between IL-4 and the expression of MCP-1.
FAU - Schimmer, R C
AU  - Schimmer RC
AD  - Department of Surgery, University of Zurich Medical School, Switzerland.
FAU - Schrier, D J
AU  - Schrier DJ
FAU - Flory, C M
AU  - Flory CM
FAU - Laemont, K D
AU  - Laemont KD
FAU - Tung, D
AU  - Tung D
FAU - Metz, A L
AU  - Metz AL
FAU - Friedl, H P
AU  - Friedl HP
FAU - Conroy, M C
AU  - Conroy MC
FAU - Warren, J S
AU  - Warren JS
FAU - Beck, B
AU  - Beck B
FAU - Ward, P A
AU  - Ward PA
LA  - eng
GR  - HL-31963/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Antibodies, Blocking)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Peptidoglycan)
RN  - 130068-27-8 (Interleukin-10)
RN  - 207137-56-2 (Interleukin-4)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Animals
MH  - Antibodies, Blocking/administration & dosage
MH  - Arthritis/etiology/*immunology/pathology
MH  - Cell Movement/immunology
MH  - Chemokine CCL2/analysis/genetics/immunology/*physiology
MH  - Female
MH  - Immunohistochemistry
MH  - In Situ Hybridization
MH  - Injections, Intra-Articular
MH  - Injections, Intravenous
MH  - Interferon-gamma/immunology/*physiology
MH  - Interleukin-10/immunology/*physiology
MH  - Interleukin-4/immunology/*physiology
MH  - Peptidoglycan/*administration & dosage
MH  - Rats
MH  - Rats, Inbred Lew
MH  - Spleen/cytology/immunology
MH  - Streptococcus/*immunology
MH  - T-Lymphocytes/pathology
EDAT- 1998/05/07 00:00
MHDA- 1998/05/07 00:01
CRDT- 1998/05/07 00:00
PHST- 1998/05/07 00:00 [pubmed]
PHST- 1998/05/07 00:01 [medline]
PHST- 1998/05/07 00:00 [entrez]
PST - ppublish
SO  - J Immunol. 1998 Feb 1;160(3):1466-71.