PMID- 9570740
OWN - NLM
STAT- MEDLINE
DCOM- 19980615
LR  - 20061115
IS  - 0196-3635 (Print)
IS  - 0196-3635 (Linking)
VI  - 19
IP  - 2
DP  - 1998 Mar-Apr
TI  - Levels of hepatocyte growth factor/scatter factor (HGF/SF) in seminal plasma of 
      patients with andrological diseases.
PG  - 175-82
AB  - Hepatocyte growth factor/scatter factor (HGF/SF) has all the characteristics of a 
      molecule suitable for functioning in regulatory networks of motility, such as the 
      spermatogenic epithelium, where spermatogenic cells must migrate between the 
      cells of Sertoli, and it exerts its effect through binding of its high-affinity 
      receptor (c-met). Considering the findings that c-met receptor is expressed in 
      the human testis and on spermatozoa, and that HGF/SF in seminal plasma consists 
      of pro-HGF/SF, mature alphabeta-HGF/SF, and less active forms of HGF/SF, we 
      investigated the concentration and biological activity of HGF/SF in seminal 
      plasma and their correlation with parameters of spermatogenesis to obtain better 
      insight into mechanisms that may be involved in the pathogenesis of male 
      infertility. We also evaluated the potential value of assessment of hepatocyte 
      growth factor concentration and its bioactivity for the diagnosis of certain 
      pathological conditions of male reproduction. We studied the concentration and 
      biological activity of HGF/SF in seminal plasma of normal men and of patients 
      with a range of andrological diseases or conditions by measuring HGF/SF in 
      seminal plasma by enzyme-linked immunosorbent assay and by scatter assay using 
      Madin-Darby canine kidney epithelial cells. We identified three sources of HGF/SF 
      in seminal plasma. In samples from vasectomized men (n = 30; 2.01 ng/ml) and in 
      split ejaculate samples (n = 6; 1e fraction 2.75 ng/ml, 2e fraction 1.62 ng/ml), 
      a prostatic origin can be certified. This HGF/SF has low biological activity 
      (133.3 U/ml). In inflammation of the accessory sex glands (n = 40), a high amount 
      of HGF/SF (3.04 ng/ml) can be generated by white blood cells and has moderate 
      scatter activity (426.7 U/ml). In normozoospermic samples, there is a lower 
      amount of HGF/SF (1.12 ng/ml), with strong scatter activity (1280.0 U/ml). 
      Finally, the clear difference between the low amount of HGF/SF (1.06 ng/ml) with 
      poor scatter activity (106.6 U/ml) in oligozoospermic samples (n = 28) and the 
      high amount of HGF/SF (3.35 ng/ml) with strong scatter activity (853.3 U/ml) in 
      samples from men with azoospermia of primary testicular failure (n = 18) suggests 
      a mainly testicular origin, with different activity in different pathological 
      conditions.
FAU - Depuydt, C E
AU  - Depuydt CE
AD  - Department of Internal Medicine, University Hospital Ghent, Belgium.
FAU - De Potter, C R
AU  - De Potter CR
FAU - Zalata, A
AU  - Zalata A
FAU - Baekelandt, E
AU  - Baekelandt E
FAU - Bosmans, E
AU  - Bosmans E
FAU - Comhaire, F H
AU  - Comhaire FH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Androl
JT  - Journal of andrology
JID - 8106453
RN  - 67256-21-7 (Hepatocyte Growth Factor)
RN  - EC 2.3.2.2 (gamma-Glutamyltransferase)
RN  - EC 3.2.1.20 (alpha-Glucosidases)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Chromatography, Affinity
MH  - Dogs
MH  - Hepatocyte Growth Factor/*metabolism
MH  - Humans
MH  - Infertility, Male/*metabolism
MH  - Male
MH  - Semen/enzymology/*metabolism
MH  - alpha-Glucosidases/metabolism
MH  - gamma-Glutamyltransferase/metabolism
EDAT- 1998/05/07 00:00
MHDA- 1998/05/07 00:01
CRDT- 1998/05/07 00:00
PHST- 1998/05/07 00:00 [pubmed]
PHST- 1998/05/07 00:01 [medline]
PHST- 1998/05/07 00:00 [entrez]
PST - ppublish
SO  - J Androl. 1998 Mar-Apr;19(2):175-82.