PMID- 9573349
OWN - NLM
STAT- MEDLINE
DCOM- 19980625
LR  - 20190707
IS  - 0378-1119 (Print)
IS  - 0378-1119 (Linking)
VI  - 211
IP  - 1
DP  - 1998 Apr 28
TI  - Tissue-specific chromatin structure at the hepatocyte growth factor/scatter 
      factor gene promoter.
PG  - 141-50
AB  - Hepatocyte growth factor/scatter factor (HGF/SF) is a recently characterised 
      molecule with many remarkable functions. Its involvement in important processes 
      such as cell proliferation, cell migration, morphogenesis and organ development 
      implies that its activity should be tightly regulated. To understand the 
      molecular mechanisms controlling HGF/SF transcription, we have analysed DNaseI 
      hypersensitive sites (DHS) along rat and human HGF/SF genes in various tissues 
      and cell types. We identified five DHS along the rat gene, two in the 5'-flanking 
      region and three in the first intron. These sites are only found in rat tissues 
      and rat cell lines, which express HGF/SF. The strongest hypersensitive site map 
      to a region that corresponds to the promoter by start site analysis. A single 
      tissue-specific DHS is present in human cell lines that express HGF/SF and 
      corresponds to the promoter region. Our results suggest that chromatin 
      accessibility plays a major role in the regulation of HGF/SF transcription 
      regulation.
FAU - Price, M
AU  - Price M
AD  - Swiss Institute for Experimental Cancer Research (ISREC), Chemin des Boveresses 
      155, CH-1066, Epalinges, Switzerland.
FAU - Fivaz, J
AU  - Fivaz J
FAU - Jotterand, A
AU  - Jotterand A
FAU - Mirkovitch, J
AU  - Mirkovitch J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Gene
JT  - Gene
JID - 7706761
RN  - 0 (Chromatin)
RN  - 0 (Interleukin-3)
RN  - 0 (RNA, Messenger)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
RN  - 9007-49-2 (DNA)
RN  - EC 3.1.21.1 (Deoxyribonuclease I)
RN  - EC 3.1.31.1 (Micrococcal Nuclease)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Chromatin/*genetics
MH  - DNA/metabolism
MH  - Deoxyribonuclease I/metabolism
MH  - Hepatocyte Growth Factor/*genetics
MH  - Humans
MH  - Interleukin-3/*chemistry
MH  - Micrococcal Nuclease/metabolism
MH  - Promoter Regions, Genetic/*genetics
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Transcription, Genetic/genetics
EDAT- 1998/05/29 00:00
MHDA- 1998/05/29 00:01
CRDT- 1998/05/29 00:00
PHST- 1998/05/29 00:00 [pubmed]
PHST- 1998/05/29 00:01 [medline]
PHST- 1998/05/29 00:00 [entrez]
AID - S0378-1119(98)00089-4 [pii]
AID - 10.1016/s0378-1119(98)00089-4 [doi]
PST - ppublish
SO  - Gene. 1998 Apr 28;211(1):141-50. doi: 10.1016/s0378-1119(98)00089-4.