PMID- 9573536
OWN - NLM
STAT- MEDLINE
DCOM- 19980630
LR  - 20071114
IS  - 0085-2538 (Print)
IS  - 0085-2538 (Linking)
VI  - 53
IP  - 5
DP  - 1998 May
TI  - Infection of human primary renal epithelial cells with HIV-1 from children with 
      HIV-associated nephropathy.
PG  - 1217-29
AB  - Children affected with human immunodefficiency virus (HIV)-associated nephropathy 
      (HIVAN) usually develop significant renal glomerular and tubular epithelial cell 
      injury. The pathogenesis of these changes is not clearly understood. Human renal 
      tubular epithelial cells (RTEc) do not express CD4 surface receptors, and it is 
      not clear whether these cells can be infected by HIV-1. Certain strains of HIV-1, 
      however, have been shown capable of infecting CD4-negative epithelial cell lines. 
      We hypothesized that the inability of laboratory strains of HIV-1 to infect renal 
      epithelial cells may be due to a limited tropism, as opposed to wild-type viruses 
      derived from children with HIVAN, and that viruses derived from these children 
      are capable of infecting RTEc from the same patient. Here, we have demonstrated 
      that HIV-1 isolates from children with HIVAN can productively infect RTEc through 
      a CD4 independent pathway, and that infected mononuclear cells can transfer the 
      virus to human RTEc. Human RTEc sustained low levels of viral replication and 
      HIV-1 inhibited the growth and survival of cultured human RTEc. Thus, HIV-1 may 
      directly induce degenerative changes in RTEc of children with HIVAN. Infected 
      macrophages may play a relevant role in this process by transferring viruses to 
      RTEc.
FAU - Ray, P E
AU  - Ray PE
AD  - Children's Research Institute, Children's National Medical Center, Washington, 
      D.C., USA. Pray@cnmc.org
FAU - Liu, X H
AU  - Liu XH
FAU - Henry, D
AU  - Henry D
FAU - Dye, L 3rd
AU  - Dye L 3rd
FAU - Xu, L
AU  - Xu L
FAU - Orenstein, J M
AU  - Orenstein JM
FAU - Schuztbank, T E
AU  - Schuztbank TE
LA  - eng
GR  - R0-1 DK49419/DK/NIDDK NIH HHS/United States
GR  - R0-1 HL55605/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Kidney Int
JT  - Kidney international
JID - 0323470
RN  - 0 (DNA, Viral)
RN  - 0 (RNA, Viral)
SB  - IM
MH  - AIDS-Associated Nephropathy/pathology/*virology
MH  - Cell Division
MH  - Cells, Cultured
MH  - Child
MH  - DNA, Viral/genetics/isolation & purification
MH  - Epithelium/ultrastructure/virology
MH  - HIV-1/*isolation & purification/*pathogenicity/physiology
MH  - Humans
MH  - In Situ Hybridization
MH  - Kidney Tubules/ultrastructure/*virology
MH  - Macrophages/ultrastructure/virology
MH  - Microscopy, Electron
MH  - RNA, Viral/genetics/isolation & purification
MH  - Virus Replication
EDAT- 1998/05/09 02:16
MHDA- 2001/03/28 10:01
CRDT- 1998/05/09 02:16
PHST- 1998/05/09 02:16 [pubmed]
PHST- 2001/03/28 10:01 [medline]
PHST- 1998/05/09 02:16 [entrez]
AID - S0085-2538(15)30530-5 [pii]
AID - 10.1046/j.1523-1755.1998.00900.x [doi]
PST - ppublish
SO  - Kidney Int. 1998 May;53(5):1217-29. doi: 10.1046/j.1523-1755.1998.00900.x.