PMID- 9574568
OWN - NLM
STAT- MEDLINE
DCOM- 19980521
LR  - 20071114
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 160
IP  - 9
DP  - 1998 May 1
TI  - Monocyte chemoattractant protein-1 synthesis by murine lung fibroblasts modulates 
      CD4+ T cell activation.
PG  - 4606-14
AB  - This study addressed the role of endogenous monocyte chemoattractant protein-1 
      (MCP-1) in Ag-stimulated lymphokine synthesis and proliferation by CD4+ T cells 
      during their coculture with purified lung fibroblasts or splenic macrophages. 
      Initial experiments showed that fibroblasts exposed to IL-4, TNF alpha, or IL-4 
      and TNF-alpha (all at 10 ng/ml) for 24 h released five- to eightfold more MCP-1 
      than similarly treated splenic macrophages. In 72-h coculture experiments, the 
      synthesis of IL-4 by OVA-activated CD4+ T cells added to lung fibroblasts or 
      splenic macrophages was significantly inhibited when endogenous MCP-1 was 
      neutralized using polyclonal anti-MCP-1 antiserum. In these same cocultures, 
      IFN-gamma levels were significantly enhanced. Similarly, IFN-gamma levels were 
      significantly enhanced in 72-h cocultures of a purified peptide 
      derivative-activated CD4+ Th1 clone and lung fibroblasts or splenic macrophages 
      following immunoneutralization of MCP-1. In separate experiments, the selective 
      inhibition of MCP-1 synthesis by lung fibroblasts and splenic macrophages using 
      an MCP-1 antisense oligonucleotide significantly enhanced the proliferation of 
      CD4+ T cells during a 96-h coculture. Taken together, these data suggest that 
      MCP-1 exerts an immunomodulatory effect on CD4+ T cell-derived IL-4 and IFN-gamma 
      release and CD4+ T cell proliferation during cell-to-cell interactions.
FAU - Hogaboam, C M
AU  - Hogaboam CM
AD  - Department of Pathology, University of Michigan Medical School, Ann Arbor 48109, 
      USA.
FAU - Lukacs, N W
AU  - Lukacs NW
FAU - Chensue, S W
AU  - Chensue SW
FAU - Strieter, R M
AU  - Strieter RM
FAU - Kunkel, S L
AU  - Kunkel SL
LA  - eng
GR  - 1P50HL56402/HL/NHLBI NIH HHS/United States
GR  - HL31963/HL/NHLBI NIH HHS/United States
GR  - HL35276/HL/NHLBI NIH HHS/United States
GR  - etc.
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Chemokine CCL2)
RN  - 0 (Oligonucleotides, Antisense)
SB  - IM
MH  - Animals
MH  - CD4-Positive T-Lymphocytes/*immunology/pathology
MH  - Cell Division/drug effects
MH  - Chemokine CCL2/biosynthesis/*immunology
MH  - Coculture Techniques
MH  - Female
MH  - Fibroblasts/immunology/metabolism/pathology
MH  - Lung/*immunology/pathology
MH  - *Lymphocyte Activation/drug effects
MH  - Mice
MH  - Mice, Inbred CBA
MH  - Oligonucleotides, Antisense/genetics/pharmacology
EDAT- 1998/05/09 00:00
MHDA- 1998/05/09 00:01
CRDT- 1998/05/09 00:00
PHST- 1998/05/09 00:00 [pubmed]
PHST- 1998/05/09 00:01 [medline]
PHST- 1998/05/09 00:00 [entrez]
PST - ppublish
SO  - J Immunol. 1998 May 1;160(9):4606-14.