PMID- 9576340
OWN - NLM
STAT- MEDLINE
DCOM- 19980616
LR  - 20190522
IS  - 1040-6387 (Print)
IS  - 1040-6387 (Linking)
VI  - 10
IP  - 2
DP  - 1998 Apr
TI  - Differentiation of a porcine reproductive and respiratory syndrome virus vaccine 
      strain from North American field strains by restriction fragment length 
      polymorphism analysis of ORF 5.
PG  - 140-4
AB  - The suitability of restriction fragment length polymorphism (RFLP) analysis for 
      differentiating a porcine reproductive and respiratory syndrome virus (PRRSV) 
      vaccine strain from other North American field strains was investigated. Open 
      reading frame 5 nucleotide sequence data of the vaccine virus, its parent strain 
      VR-2332, and 22 other strains of PRRSV included in this study indicated that 3 
      restriction enzyme gel patterns characterize the vaccine virus and the parent 
      strain genotype. The combined 3 RFLP patterns differentiate the vaccine and 
      parent virus from other PRRSV strains. This test will be a valuable tool in 
      epidemiologic studies and will be useful in identifying individual strains in 
      cases of multistrain PRRSV infections.
FAU - Wesley, R D
AU  - Wesley RD
AD  - Virology Swine Research Unit, National Animal Disease Center, USDA, Agricultural 
      Research Service, Ames, IA 50010, USA.
FAU - Mengeling, W L
AU  - Mengeling WL
FAU - Lager, K M
AU  - Lager KM
FAU - Clouser, D F
AU  - Clouser DF
FAU - Landgraf, J G
AU  - Landgraf JG
FAU - Frey, M L
AU  - Frey ML
LA  - eng
SI  - GENBANK/AF020048
SI  - GENBANK/AF020049
SI  - GENBANK/AF020050
PT  - Journal Article
PL  - United States
TA  - J Vet Diagn Invest
JT  - Journal of veterinary diagnostic investigation : official publication of the 
      American Association of Veterinary Laboratory Diagnosticians, Inc
JID - 9011490
RN  - 0 (Codon)
RN  - 0 (RNA, Viral)
RN  - 0 (Viral Vaccines)
RN  - EC 3.1.21.- (DNA Restriction Enzymes)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Codon
MH  - DNA Restriction Enzymes
MH  - North America
MH  - *Open Reading Frames
MH  - Phylogeny
MH  - Polymerase Chain Reaction
MH  - *Polymorphism, Restriction Fragment Length
MH  - Porcine Reproductive and Respiratory Syndrome/*immunology/prevention & control
MH  - Porcine respiratory and reproductive syndrome 
      virus/classification/*genetics/*immunology
MH  - RNA, Viral/analysis
MH  - Swine
MH  - *Viral Vaccines
EDAT- 1998/05/12 00:00
MHDA- 1998/05/12 00:01
CRDT- 1998/05/12 00:00
PHST- 1998/05/12 00:00 [pubmed]
PHST- 1998/05/12 00:01 [medline]
PHST- 1998/05/12 00:00 [entrez]
AID - 10.1177/104063879801000204 [doi]
PST - ppublish
SO  - J Vet Diagn Invest. 1998 Apr;10(2):140-4. doi: 10.1177/104063879801000204.