PMID- 9577344
OWN - NLM
STAT- MEDLINE
DCOM- 19980514
LR  - 20190501
IS  - 0017-5749 (Print)
IS  - 1458-3288 (Electronic)
IS  - 0017-5749 (Linking)
VI  - 42
IP  - 3
DP  - 1998 Mar
TI  - Mucosal subepithelial binding sites for the bacterial chemotactic peptide, 
      formyl-methionyl-leucyl-phenylalanine (FMLP).
PG  - 374-9
AB  - BACKGROUND: The bacterial chemotactic peptide 
      N-formyl-methionine-leucine-phenylalanine (FMLP) is produced by enteric flora and 
      is one of the factors implicated in contributing to inflammatory bowel disease. 
      Expression of receptors for FMLP on human phagocytes (polymorphs and monocytes) 
      is well established, but there is conflicting evidence regarding the potential 
      expression of FMLP receptors on other cells within the mucosa, particularly the 
      epithelial cells. AIMS: To map FMLP receptors within intestinal mucosa using 
      several different experimental approaches. METHODS AND RESULTS: Radioligand 
      binding assays with 'H-FMLP' revealed no specific binding to primary cultured 
      colonic enterocytes or to the cell line HT29, whereas neutrophils, as expected, 
      exhibited specific binding with a Kd of 19 nM and approximately 2 x 10(4) 
      receptors per cell. FITC labelled FMLP exhibited specific, displaceable binding 
      on flow cytometry to neutrophils and monocytes but not to 10 gastrointestinal 
      epithelial cell lines. Isolated lamina propria lymphocytes and peripheral blood 
      lymphocytes exhibited no binding. To confirm the absence of receptors on 
      epithelia, reverse transcription polymerase chain reaction for mRNA for the 
      classic FMLP receptor was performed. While the presence of message was detected 
      in activated peripheral blood phagocytes, it was not detected in epithelial cell 
      lines. To exclude the possibility of FMLP binding to other receptors such as 
      tachykinin receptors on epithelia, FITC labelled FMLP binding in tissue sections 
      confirmed that the binding is subepithelial--that is, in the lamina propria. 
      CONCLUSION: Receptors for FMLP are subepithelial and map to the lamina propria of 
      the gastrointestinal mucosa.
FAU - Anton, P
AU  - Anton P
AD  - Department of Medicine, National University of Ireland, Cork, Republic of 
      Ireland.
FAU - O'Connell, J
AU  - O'Connell J
FAU - O'Connell, D
AU  - O'Connell D
FAU - Whitaker, L
AU  - Whitaker L
FAU - O'Sullivan, G C
AU  - O'Sullivan GC
FAU - Collins, J K
AU  - Collins JK
FAU - Shanahan, F
AU  - Shanahan F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Gut
JT  - Gut
JID - 2985108R
RN  - 0 (Bacterial Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Formyl Peptide)
RN  - 0 (Receptors, Immunologic)
RN  - 0 (Receptors, Peptide)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
SB  - IM
MH  - Animals
MH  - Bacterial Proteins/*metabolism
MH  - Cells, Cultured
MH  - Colon
MH  - Flow Cytometry
MH  - HT29 Cells
MH  - Humans
MH  - Intestinal Mucosa/*chemistry/metabolism
MH  - Monocytes/metabolism
MH  - N-Formylmethionine Leucyl-Phenylalanine/*metabolism
MH  - Neutrophils/metabolism
MH  - Polymerase Chain Reaction
MH  - RNA, Messenger/analysis
MH  - Rabbits
MH  - Radioligand Assay
MH  - Receptors, Formyl Peptide
MH  - Receptors, Immunologic/*analysis
MH  - Receptors, Peptide/*analysis
PMC - PMC1727033
EDAT- 1998/05/13 00:00
MHDA- 1998/05/13 00:01
PMCR- 2001/03/01
CRDT- 1998/05/13 00:00
PHST- 1998/05/13 00:00 [pubmed]
PHST- 1998/05/13 00:01 [medline]
PHST- 1998/05/13 00:00 [entrez]
PHST- 2001/03/01 00:00 [pmc-release]
AID - 10.1136/gut.42.3.374 [doi]
PST - ppublish
SO  - Gut. 1998 Mar;42(3):374-9. doi: 10.1136/gut.42.3.374.