PMID- 9578419
OWN - NLM
STAT- MEDLINE
DCOM- 19980609
LR  - 20071114
IS  - 0960-8931 (Print)
IS  - 0960-8931 (Linking)
VI  - 7 Suppl 2
DP  - 1997 Aug
TI  - Beta2-microglobulin gene mutations in human melanoma cells: molecular 
      characterization and implications for immune surveillance.
PG  - S67-74
AB  - In recent years, the mechanisms underlying defects in the expression/function of 
      human leukocyte antigen (HLA) class I antigens have been analyzed in an 
      increasing number of melanoma cells, since these abnormalities are likely to have 
      a negative impact on T-cell-based immunotherapy of melanoma. This article reviews 
      the information about the molecular defects found in melanoma cell lines that do 
      not express HLA class I antigens, following a concise description of the 
      structure and assembly of HLA class I antigens. Distinct defects ranging from 
      large deletions to point mutations in beta2-microglobulin genes have been found 
      in melanoma cells. A mutation in an 8 base-pair CT repeat region of exon 1 has 
      been found frequently in melanoma cell lines suggesting that this region of the 
      beta2-microglobulin gene is a hot-spot for mutations. The effects of 
      beta2-microglobulin mutations are mostly at the level of translation, emphasizing 
      the importance of analyzing beta2-microglobulin expression at the protein level 
      in melanoma lesions without detectable HLA class I antigen expression. 
      Interestingly, many melanoma cell lines have additional defects that directly 
      impact HLA class I antigen expression. Therefore, multiple mechanisms that affect 
      the expression/function of HLA class I antigens appear to be available to 
      melanoma cells to escape from immune recognition.
FAU - Hicklin, D J
AU  - Hicklin DJ
AD  - Department of Microbiology and Immunology, New York Medical College, Valhalla 
      10595, USA.
FAU - Dellaratta, D V
AU  - Dellaratta DV
FAU - Kishore, R
AU  - Kishore R
FAU - Liang, B
AU  - Liang B
FAU - Kageshita, T
AU  - Kageshita T
FAU - Ferrone, S
AU  - Ferrone S
LA  - eng
GR  - CA51814/CA/NCI NIH HHS/United States
GR  - CA67108/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PT  - Review
PL  - England
TA  - Melanoma Res
JT  - Melanoma research
JID - 9109623
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (beta 2-Microglobulin)
SB  - IM
MH  - Exons
MH  - Histocompatibility Antigens Class I/biosynthesis/immunology
MH  - Humans
MH  - Immunologic Surveillance/*physiology
MH  - Melanoma/*genetics/*immunology
MH  - *Point Mutation
MH  - beta 2-Microglobulin/*genetics/*immunology
RF  - 47
EDAT- 1997/08/01 00:00
MHDA- 1998/05/13 00:01
CRDT- 1997/08/01 00:00
PHST- 1997/08/01 00:00 [pubmed]
PHST- 1998/05/13 00:01 [medline]
PHST- 1997/08/01 00:00 [entrez]
PST - ppublish
SO  - Melanoma Res. 1997 Aug;7 Suppl 2:S67-74.