PMID- 9579843
OWN - NLM
STAT- MEDLINE
DCOM- 19980527
LR  - 20231024
IS  - 0007-0920 (Print)
IS  - 1532-1827 (Electronic)
IS  - 0007-0920 (Linking)
VI  - 77
IP  - 8
DP  - 1998 Apr
TI  - Chromosomal imbalances in primary and metastatic pancreatic carcinoma as detected 
      by interphase cytogenetics: basic findings and clinical aspects.
PG  - 1337-42
AB  - To date, cytogenetic studies on pancreatic carcinoma are rare, and little is 
      known about the frequency of cytogenetic aberrations in primary carcinomas 
      compared with metastatic tumour cells. We therefore evaluated the frequency of 
      chromosomal aberrations in 12 primary pancreatic carcinomas and in effusion 
      specimens from 25 patients with pancreatic cancer by using interphase 
      fluorescence in situ hybridization (FISH) and a panel of four centromeric probes. 
      Hyperdiploidy and chromosomal imbalances, predominantly affecting chromosome 8, 
      were a constant finding in metastatic effusion cells, whereas concordant gain of 
      chromosomes or relative loss of chromosome 18 characterized primary pancreatic 
      carcinomas. The potential role of oncogenes located on chromosome 8 for 
      pancreatic cancer progression was further investigated by double-hybridization 
      studies of aneuploid effusion cells with a probe to 8q24 (MYC) and a centromeric 
      probe to chromosome 8, which demonstrated amplification of the MYC oncogene in 
      two of ten cases (20%). Finally, a potential application of basic findings in the 
      clinical setting was tested by searching for micrometastatic cells in effusions 
      from pancreatic cancer patients primarily negative by FISH. Two-colour FISH in 
      combination with extensive screening (>10,000 nuclei) seems to be a useful tool 
      to unequivocally identify micrometastatic cells by demonstrating hyperdiploidy 
      and intranuclear chromosomal heterogeneity.
FAU - Zojer, N
AU  - Zojer N
AD  - First Department of Internal Medicine, University of Vienna, Austria.
FAU - Fiegl, M
AU  - Fiegl M
FAU - Mullauer, L
AU  - Mullauer L
FAU - Chott, A
AU  - Chott A
FAU - Roka, S
AU  - Roka S
FAU - Ackermann, J
AU  - Ackermann J
FAU - Raderer, M
AU  - Raderer M
FAU - Kaufmann, H
AU  - Kaufmann H
FAU - Reiner, A
AU  - Reiner A
FAU - Huber, H
AU  - Huber H
FAU - Drach, J
AU  - Drach J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Br J Cancer
JT  - British journal of cancer
JID - 0370635
SB  - IM
MH  - Ascites/genetics/pathology
MH  - *Chromosome Aberrations
MH  - Chromosomes, Human, Pair 18/*genetics
MH  - Chromosomes, Human, Pair 8/*genetics
MH  - Cytogenetics/methods
MH  - Gene Amplification
MH  - Genes, myc/genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Interphase/*genetics
MH  - Pancreatic Neoplasms/*genetics/*secondary
MH  - Pleural Effusion/genetics/pathology
PMC - PMC2150163
EDAT- 1998/05/14 00:00
MHDA- 1998/05/14 00:01
CRDT- 1998/05/14 00:00
PHST- 1998/05/14 00:00 [pubmed]
PHST- 1998/05/14 00:01 [medline]
PHST- 1998/05/14 00:00 [entrez]
AID - 10.1038/bjc.1998.223 [doi]
PST - ppublish
SO  - Br J Cancer. 1998 Apr;77(8):1337-42. doi: 10.1038/bjc.1998.223.