PMID- 9580790
OWN - NLM
STAT- MEDLINE
DCOM- 19980521
LR  - 20240109
IS  - 0007-0963 (Print)
IS  - 0007-0963 (Linking)
VI  - 138
IP  - 3
DP  - 1998 Mar
TI  - Minoxidil upregulates the expression of vascular endothelial growth factor in 
      human hair dermal papilla cells.
PG  - 407-11
AB  - The hair follicle dermal papilla which controls hair growth, is characterized in 
      the anagen phase by a highly developed vascular network. We have demonstrated in 
      a previous study that the expression of an angiogenic growth factor called 
      vascular endothelial growth factor (VEGF) mRNA varied during the hair cycle. VEGF 
      mRNA is strongly expressed in dermal papilla cells (DPC) in the anagen phase, but 
      during the catagen and telogen phases. VEGF mRNA is less strongly expressed. This 
      involvement of VEGF during the hair cycle allowed us to determine whether VEGF 
      mRNA expression by DPC was regulated by minoxidil. In addition, the effect of 
      minoxidil on VEGF protein synthesis in both cell extracts and DPC-conditioned 
      medium, was investigated immunoenzymatically. Both VEGF mRNA and protein were 
      significantly elevated in treated DPC compared with controls. DPC incubated with 
      increasing minoxidil concentrations (0.2, 2, 6, 12 and 24 mumol/L) induced a 
      dose-dependent expression of VEGF mRNA. Quantification of transcripts showed that 
      DPC stimulated with 24 mumol/L minoxidil express six times more VEGF mRNA than 
      controls. Similarly, VEGF protein production increases in cell extracts and 
      conditioned media following minoxidil stimulation. These studies strongly support 
      the likely involvement of minoxidil in the development of dermal papilla 
      vascularization via a stimulation of VEGF expression, and support the hypothesis 
      that minoxidil has a physiological role in maintaining a good vascularization of 
      hair follicles in androgenetic alopecia.
FAU - Lachgar, S
AU  - Lachgar S
AD  - Laboratoire de Biologie Cellulaire Cutanee, Institut de Recherche Pierre Fabre, 
      Faculte de Medecine Rangueil, Toulouse, France.
FAU - Charveron, M
AU  - Charveron M
FAU - Gall, Y
AU  - Gall Y
FAU - Bonafe, J L
AU  - Bonafe JL
LA  - eng
PT  - Journal Article
PL  - England
TA  - Br J Dermatol
JT  - The British journal of dermatology
JID - 0004041
RN  - 0 (Endothelial Growth Factors)
RN  - 0 (Lymphokines)
RN  - 0 (RNA, Messenger)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - 0 (Vascular Endothelial Growth Factors)
RN  - 0 (Vasodilator Agents)
RN  - 5965120SH1 (Minoxidil)
SB  - IM
MH  - Cell Culture Techniques
MH  - Dose-Response Relationship, Drug
MH  - Endothelial Growth Factors/genetics/metabolism/*pharmacology
MH  - Hair Follicle/*drug effects/metabolism
MH  - Humans
MH  - In Situ Hybridization
MH  - Lymphokines/*drug effects/genetics/metabolism/*pharmacology
MH  - Microscopy, Confocal
MH  - Minoxidil/*pharmacology
MH  - RNA, Messenger/genetics
MH  - Up-Regulation/*drug effects
MH  - Vascular Endothelial Growth Factor A
MH  - Vascular Endothelial Growth Factors
MH  - Vasodilator Agents/*pharmacology
EDAT- 1998/05/15 00:00
MHDA- 1998/05/15 00:01
CRDT- 1998/05/15 00:00
PHST- 1998/05/15 00:00 [pubmed]
PHST- 1998/05/15 00:01 [medline]
PHST- 1998/05/15 00:00 [entrez]
AID - 10.1046/j.1365-2133.1998.02115.x [doi]
PST - ppublish
SO  - Br J Dermatol. 1998 Mar;138(3):407-11. doi: 10.1046/j.1365-2133.1998.02115.x.