PMID- 9586116 OWN - NLM STAT- MEDLINE DCOM- 19980723 LR - 20001218 IS - 0261-3166 (Print) IS - 0261-3166 (Linking) IP - 37 DP - 1997 TI - Recognition system of class II tRNA in Escherichia coli and yeast. PG - 295-6 AB - The recognition system of class II tRNA, tRNA(Ser) and tRNA(Leu), in a yeast Saccharomyces cerevisiae was studied using T7 RNA polymerase transcription system. Yeast SerRS recognizes the long variable arm as in E. coli. However, the anticodon loop of tRNA(Leu), which has no effect on leucylation in E. coli, play a key role for recognition by LeuRS. Results suggest that the recognition style of yeast class II tRNA is substantially different from that of E. coli. FAU - Soma, A AU - Soma A AD - Department of Biology, Faculty of Science, Hirosaki University, Aomori, Japan. FAU - Himeno, H AU - Himeno H LA - eng PT - Journal Article PL - England TA - Nucleic Acids Symp Ser JT - Nucleic acids symposium series JID - 8007206 RN - 0 (RNA, Bacterial) RN - 0 (RNA, Fungal) RN - 0 (RNA, Transfer, Leu) RN - 0 (RNA, Transfer, Ser) SB - IM MH - Escherichia coli/*genetics MH - RNA, Bacterial/genetics MH - RNA, Fungal/genetics MH - RNA, Transfer, Leu/*genetics MH - RNA, Transfer, Ser/*genetics MH - Saccharomyces cerevisiae/*genetics MH - Species Specificity EDAT- 1997/01/01 00:00 MHDA- 1998/05/20 00:01 CRDT- 1997/01/01 00:00 PHST- 1997/01/01 00:00 [pubmed] PHST- 1998/05/20 00:01 [medline] PHST- 1997/01/01 00:00 [entrez] PST - ppublish SO - Nucleic Acids Symp Ser. 1997;(37):295-6.