PMID- 9588355
OWN - NLM
STAT- MEDLINE
DCOM- 19980527
LR  - 20131121
IS  - 0033-7587 (Print)
IS  - 0033-7587 (Linking)
VI  - 149
IP  - 5
DP  - 1998 May
TI  - Rejoining and misrejoining of radiation-induced chromatin breaks. IV. Charged 
      particles.
PG  - 446-54
AB  - We have recently reported the kinetics of chromosome rejoining and exchange 
      formation in human lymphocytes exposed to gamma rays using the techniques of 
      fluorescence in situ hybridization (FISH) and premature chromosome condensation 
      (PCC). In this paper, we have extended previous measurements to cells exposed to 
      charged particles. Our goal was to determine differences in chromatin break 
      rejoining and misrejoining after exposure to low- and high-linear energy transfer 
      (LET) radiation. Cells were irradiated with hydrogen, neon, carbon or iron ions 
      in the LET range 0.3-140 keV/microm and were incubated at 37 degrees C for 
      various times after exposure. Little difference was observed in the yield of 
      early prematurely condensed chromosome breaks for the different ions. The 
      kinetics of break rejoining was exponential for all ions and had similar time 
      constants, but the residual level of unrejoined breaks after prolonged incubation 
      was higher for high-LET radiation. The kinetics of exchange formation was also 
      similar for the different ions, but the yield of chromosome interchanges measured 
      soon after exposure was higher for high-LET particles, suggesting that a higher 
      fraction of DNA breaks are misrejoined quickly. On the other hand, the rate of 
      formation of complete exchanges was slightly lower for densely ionizing 
      radiation. The ratios between the yields of different types of aberrations 
      observed at 10 h postirradiation in prematurely condensed chromosome preparations 
      were dependent on LET. We found significant differences between the yields of 
      aberrations measured in interphase (after repair) and metaphase for densely 
      ionizing radiation. This difference might be caused by prolonged mitotic delay 
      and/or interphase death. Overall, the results point out significant differences 
      between low- and high-LET radiation for the formation of chromosome aberrations.
FAU - Durante, M
AU  - Durante M
AD  - Dipartimento di Scienze Fisiche, Universita Federico II, Monte S. Angelo, Napoli, 
      Italy.
FAU - Furusawa, Y
AU  - Furusawa Y
FAU - George, K
AU  - George K
FAU - Gialanella, G
AU  - Gialanella G
FAU - Greco, O
AU  - Greco O
FAU - Grossi, G
AU  - Grossi G
FAU - Matsufuji, N
AU  - Matsufuji N
FAU - Pugliese, M
AU  - Pugliese M
FAU - Yang, T C
AU  - Yang TC
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Radiat Res
JT  - Radiation research
JID - 0401245
RN  - 0 (Cations)
RN  - 0 (Chromatin)
RN  - 7440-44-0 (Carbon)
RN  - 7YNJ3PO35Z (Hydrogen)
RN  - E1UOL152H7 (Iron)
SB  - IM
MH  - Animals
MH  - CHO Cells
MH  - Carbon
MH  - Cations
MH  - Chromatin/*radiation effects
MH  - *Chromosome Aberrations
MH  - Cricetinae
MH  - Humans
MH  - Hydrogen
MH  - In Situ Hybridization, Fluorescence
MH  - Iron
MH  - Linear Energy Transfer
MH  - Male
OTO - NASA
OT  - NASA Center JSC
OT  - NASA Discipline Radiation Health
FIR - Yang, T C
IR  - Yang TC
IRAD- JSC
EDAT- 1998/05/20 00:00
MHDA- 1998/05/20 00:01
CRDT- 1998/05/20 00:00
PHST- 1998/05/20 00:00 [pubmed]
PHST- 1998/05/20 00:01 [medline]
PHST- 1998/05/20 00:00 [entrez]
PST - ppublish
SO  - Radiat Res. 1998 May;149(5):446-54.