PMID- 9590805
OWN - NLM
STAT- MEDLINE
DCOM- 19980526
LR  - 20041117
IS  - 0412-4081 (Print)
IS  - 0412-4081 (Linking)
VI  - 32
IP  - 6
DP  - 1996 Nov
TI  - A molecular pathologic study on apoptosis in retinoblastoma and the mechanism of 
      spontaneous regression in retinoblastoma.
PG  - 405-16
AB  - OBJECTIVE: The present study was designed to prove the existence of apoptosis in 
      retinoblastoma (Rb) and to determine the pathogenic mechanism of spontaneous 
      regression of Rb as well as the relationship between them. METHODS: Qualitative 
      morphological study on Rb was performed by means of light microscope, electron 
      microscope and TdT mediated biotin-dUTP nick-end labeling (TUNEL). Quantitative 
      study was performed by automatic image analysis technology (AIAT) stained with 
      Feulgen reaction. RESULTS: The characteristic regressed area occurred in all 47 
      cases of Rb. Morphological changes observed within Rb closely resembled the 
      apoptotic cell described by Kerr et al in 1972. Under electron microscope, 
      details of apoptosis were observed in 7 cases of Rb: the morphological sequence 
      of events occurred in and around the cell nucleus. The morphology of the TUNEL 
      labeling Rb cell was various. TUNEL labeling showed more positive cells in 
      regressed area, while fewer in advanced area. AIAT revealed that apoptosis index 
      (AI) in regressed areas was higher than that in other areas of Rb, DNA average 
      ploidy (DP) consisted with the histology grade of Rb, the degree of hyperdiploid 
      (DH) in metastatic area was distinctly higher than that in other areas (P < 
      0.05). CONCLUSIONS 1. Morphologic evidence proved the existence of apoptosis, 
      especially more in regressed area of Rb. 2. Apoptosis may participate in the 
      spontaneous regression of Rb. Apoptosis contributed to the spontaneous regression 
      of Rb. 3. Tumor growth parameters of Rb (AI, DP, DH) obtained by AIAT may be used 
      as quantitative index for pathologic classification, the selection of clinical 
      treatment and the prognostic evaluation.
FAU - Mao, J
AU  - Mao J
AD  - Beijing Institute of Ophthalmology.
FAU - Sun, X
AU  - Sun X
FAU - Tian, Y
AU  - Tian Y
FAU - Li, L
AU  - Li L
FAU - Li, B
AU  - Li B
FAU - Ding, H
AU  - Ding H
FAU - Xing, H
AU  - Xing H
LA  - chi
LA  - eng
PT  - Journal Article
PL  - China
TA  - Zhonghua Yan Ke Za Zhi
JT  - [Zhonghua yan ke za zhi] Chinese journal of ophthalmology
JID - 16210540R
SB  - IM
MH  - *Apoptosis
MH  - Humans
MH  - Image Processing, Computer-Assisted
MH  - Neoplasm Regression, Spontaneous/*pathology
MH  - Retinal Neoplasms/*pathology/physiopathology
MH  - Retinoblastoma/*pathology/physiopathology
EDAT- 1996/11/01 00:00
MHDA- 1998/05/20 00:01
CRDT- 1996/11/01 00:00
PHST- 1996/11/01 00:00 [pubmed]
PHST- 1998/05/20 00:01 [medline]
PHST- 1996/11/01 00:00 [entrez]
PST - ppublish
SO  - Zhonghua Yan Ke Za Zhi. 1996 Nov;32(6):405-16.