PMID- 9591133
OWN - NLM
STAT- MEDLINE
DCOM- 19980721
LR  - 20180928
IS  - 0736-6205 (Print)
IS  - 0736-6205 (Linking)
VI  - 24
IP  - 5
DP  - 1998 May
TI  - Quick-FISH: a rapid fluorescence in situ hybridization technique for molecular 
      cytogenetic analysis.
PG  - 826-30
AB  - A rapid and simplified fluorescence in situ hybridization (FISH) technique for 
      the detection of human chromosome-specific centromeric probes is described. Using 
      chromosomes 1-, 4-, 11- and Y-specific fluorescence-labeled probes, the modified, 
      or quick-FISH technique, was compared to two conventional FISH methods. The 
      modified FISH technique detected human chromosomes without sacrificing 
      sensitivity or signal quality in both fresh-frozen and paraffin-embedded tissue 
      sections. Further studies demonstrated that this technique can be used with 
      simultaneous application of dual-color probes. This novel technique offers the 
      advantages of being simpler to perform and faster than conventional techniques. 
      The quick-FISH technique can be substituted for any sensitive conventional FISH 
      method for molecular cytogenetic analysis in fresh, fresh-frozen or 
      paraffin-embedded tissues.
FAU - Banerjee, S K
AU  - Banerjee SK
AD  - Molecular Gastroenterology & Pancreatic Cancer Research Unit, V.A. Medical 
      Center, Kansas City, MO 64128-2226, USA.
FAU - Weston, A P
AU  - Weston AP
FAU - Persons, D L
AU  - Persons DL
FAU - Campbell, D R
AU  - Campbell DR
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Biotechniques
JT  - BioTechniques
JID - 8306785
RN  - 0 (DNA Probes)
SB  - IM
MH  - Aneuploidy
MH  - Centromere/chemistry
MH  - Chromosome Aberrations/*diagnosis/*genetics/pathology
MH  - Chromosome Disorders
MH  - DNA Probes
MH  - Gastric Mucosa/chemistry/pathology
MH  - Gene Dosage
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Interphase/genetics
MH  - Sensitivity and Specificity
MH  - Time Factors
EDAT- 1998/05/20 00:00
MHDA- 1998/05/20 00:01
CRDT- 1998/05/20 00:00
PHST- 1998/05/20 00:00 [pubmed]
PHST- 1998/05/20 00:01 [medline]
PHST- 1998/05/20 00:00 [entrez]
AID - 10.2144/98245dt03 [doi]
PST - ppublish
SO  - Biotechniques. 1998 May;24(5):826-30. doi: 10.2144/98245dt03.