PMID- 9591711
OWN - NLM
STAT- MEDLINE
DCOM- 19980630
LR  - 20071114
IS  - 0889-2229 (Print)
IS  - 0889-2229 (Linking)
VI  - 14
IP  - 7
DP  - 1998 May 1
TI  - Plasma virus load evaluation in relation to disease progression in HIV-infected 
      children.
PG  - 571-7
AB  - The objective of this study was to investigate the relationship of plasma HIV RNA 
      load with survival and disease progression in HIV-infected children and to 
      determine its correlation with cellular HIV DNA. Virus load (VL, HIV RNA 
      copies/ml) was determined retrospectively by nucleic acid sequence-based 
      amplification (NASBA) assay in 144 stored plasma samples between birth and 48 
      months in 50 children of whom 40 are alive (age range, 2-13 years). On the basis 
      of clinical and immunologic status children were classified as rapid progressors 
      (RPs), or nonrapid progressors (NRPs). Proviral HIV DNA quantitated by QC-PCR 
      (quantitative competitive polymerase chain reaction) in 24 children was compared 
      with plasma HIV RNA. At age <3 months, plasma VL <750,000 copies/ml was 
      associated with significantly higher survival to age >2 years (p < or =0.01) 
      compared with a VL of > or =750,000 copies/ml. Increasing mortality was observed 
      with increasing plasma HIV RNA levels at ages 3-24 months and baseline VL of 
      infants who died before age 24 months was significantly higher (p = 0.004) than 
      baseline VL of those who survived beyond 24 months. Although baseline VL in 
      infants classified as RPs was higher than that of NRPs, the difference was not 
      statistically significant. Among surviving children 2-13 years of age, the 
      baseline VL obtained at <24 months of age was not predictive of disease severity. 
      Although no significant correlation was noted between plasma HIV RNA and proviral 
      DNA, the concurrence of positive and negative results was >80%. We conclude that 
      high plasma HIV RNA in infancy is associated with increased mortality.
FAU - Tetali, S
AU  - Tetali S
AD  - Department of Pediatrics, North Shore University Hospital-New York University 
      School of Medicine, Manhasset 11030, USA.
FAU - Bakshi, S
AU  - Bakshi S
FAU - Than, S
AU  - Than S
FAU - Pahwa, S
AU  - Pahwa S
FAU - Abrams, E
AU  - Abrams E
FAU - Romano, J
AU  - Romano J
FAU - Pahwa, S G
AU  - Pahwa SG
LA  - eng
GR  - AI 28281/AI/NIAID NIH HHS/United States
GR  - DA 05161/DA/NIDA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - AIDS Res Hum Retroviruses
JT  - AIDS research and human retroviruses
JID - 8709376
RN  - 0 (DNA, Viral)
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Adolescent
MH  - Age Factors
MH  - Child
MH  - Child, Preschool
MH  - DNA, Viral
MH  - Disease Progression
MH  - HIV Infections/mortality/*physiopathology/*virology
MH  - HIV-1/*genetics
MH  - Humans
MH  - Leukocytes, Mononuclear/virology
MH  - RNA, Viral/blood
MH  - Retrospective Studies
MH  - *Viral Load
EDAT- 1998/05/20 00:00
MHDA- 1998/05/20 00:01
CRDT- 1998/05/20 00:00
PHST- 1998/05/20 00:00 [pubmed]
PHST- 1998/05/20 00:01 [medline]
PHST- 1998/05/20 00:00 [entrez]
AID - 10.1089/aid.1998.14.571 [doi]
PST - ppublish
SO  - AIDS Res Hum Retroviruses. 1998 May 1;14(7):571-7. doi: 10.1089/aid.1998.14.571.