PMID- 9593263
OWN - NLM
STAT- MEDLINE
DCOM- 19980529
LR  - 20190914
IS  - 0887-6924 (Print)
IS  - 0887-6924 (Linking)
VI  - 12
IP  - 5
DP  - 1998 May
TI  - Multilineage involvement of Philadelphia chromosome positive acute lymphoblastic 
      leukemia.
PG  - 666-74
AB  - Acute lymphocytic leukemia (ALL) is considered a clonal disease restricted to the 
      lymphoid compartment. The Philadelphia chromosome (Ph) is found in a subset of 
      ALL with poor prognosis. Here we present the largest series of Ph+ ALL analyzed 
      for involvement of the myeloid compartment. For the first time at a single cell 
      level the presence of Ph in lineages other than lymphoid is demonstrated. 
      Granulocytes from nine patients diagnosed with BCR-ABL + ALL (eight Ph+, one Ph-) 
      were purified using two layer density gradient separation. They were further 
      identified by the morphology of DAPI-stained nuclei and studied for the presence 
      of the Ph by fluorescence in situ hybridization (FISH) using a BCR-ABL dual-color 
      probe. Ph was demonstrated in 30 to 93% of granulocytes in all patients. FISH 
      identified major and minor BCR gene breakpoints (M-bcr and m-bcr). In one 
      patient, with CD19+/34+/33-/2-/3-/7-/10- lymphoblasts, involvement of B cells 
      (CD19+), T cells (CD3+), myeloid (CD13+), erythroid (glycophorin A+) cells was 
      found by FISH following fluorescence-activated cell sorting (FACS). The diagnosis 
      of ALL as opposed to lymphoblastic transformation of CML was established based on 
      clinical and laboratory data including Western blot results demonstrating the 
      presence of p190/m-bcr in five of the nine cases studied. Results suggest that 
      Ph+ ALL originates from a pluripotent stem cell.
FAU - Schenk, T M
AU  - Schenk TM
AD  - Department of Hematology, The University of Texas MD Anderson Cancer Center, 
      Houston, USA.
FAU - Keyhani, A
AU  - Keyhani A
FAU - Bottcher, S
AU  - Bottcher S
FAU - Kliche, K O
AU  - Kliche KO
FAU - Goodacre, A
AU  - Goodacre A
FAU - Guo, J Q
AU  - Guo JQ
FAU - Arlinghaus, R B
AU  - Arlinghaus RB
FAU - Kantarjian, H M
AU  - Kantarjian HM
FAU - Andreeff, M
AU  - Andreeff M
LA  - eng
GR  - CA 16672/CA/NCI NIH HHS/United States
GR  - CA 49639/CA/NCI NIH HHS/United States
GR  - P01CA 55164/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Leukemia
JT  - Leukemia
JID - 8704895
RN  - EC 2.7.10.2 (Fusion Proteins, bcr-abl)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Blotting, Western
MH  - Female
MH  - Fusion Proteins, bcr-abl/metabolism
MH  - Granulocytes/metabolism/ultrastructure
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Male
MH  - Middle Aged
MH  - Philadelphia Chromosome
MH  - Precursor Cell Lymphoblastic Leukemia-Lymphoma/*genetics
EDAT- 1998/05/21 00:00
MHDA- 1998/05/21 00:01
CRDT- 1998/05/21 00:00
PHST- 1998/05/21 00:00 [pubmed]
PHST- 1998/05/21 00:01 [medline]
PHST- 1998/05/21 00:00 [entrez]
AID - 10.1038/sj.leu.2400986 [doi]
PST - ppublish
SO  - Leukemia. 1998 May;12(5):666-74. doi: 10.1038/sj.leu.2400986.