PMID- 9593466
OWN - NLM
STAT- MEDLINE
DCOM- 19980603
LR  - 20190826
IS  - 0145-2126 (Print)
IS  - 0145-2126 (Linking)
VI  - 22
IP  - 2
DP  - 1998 Feb
TI  - Benzene metabolites induce the loss and long arm deletion of chromosomes 5 and 7 
      in human lymphocytes.
PG  - 105-13
AB  - Two of the most common cytogenetic changes in therapy- and chemically-related 
      leukemia are the loss and long (q) arm deletions of chromosomes 5 and 7 (i.e. -5, 
      -7, del(5q) and del(7q)). We have used a novel fluorescence in situ hybridization 
      (FISH) procedure to determine if the benzene metabolites hydroquinone (HQ) and 
      1,2,4-benzenetriol (BT) can induce these specific changes in human lymphocytes 
      cultured as whole blood. Metaphase spreads were prepared and hybridized with 
      centromeric probes for chromosomes 1, 5 and 7 and sequence specific probes for 
      5q31 and 7q36-qter. HQ and BT significantly increased monosomy 5 and 7 by 3-5 
      fold (p < 0.0001). Both HQ and BT also significantly increased the rate of 
      del(5q) and del(7q) by 8-12 fold (p < 0.0001). Chromosome 7 was especially 
      susceptible to aneusomy induction by HQ and BT at low doses. These results show 
      that metabolites of benzene are highly effective in inducing changes in 
      chromosomes 5 and 7 that are involved in the development of myeloid leukemia.
FAU - Zhang, L
AU  - Zhang L
AD  - Division of Environmental Health Sciences, School of Public Health, University of 
      California, Berkeley 94720-7360, USA.
FAU - Wang, Y
AU  - Wang Y
FAU - Shang, N
AU  - Shang N
FAU - Smith, M T
AU  - Smith MT
LA  - eng
GR  - P42 ES004705/ES/NIEHS NIH HHS/United States
GR  - P42-ES04705/ES/NIEHS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Leuk Res
JT  - Leukemia research
JID - 7706787
RN  - 0 (Hydroquinones)
RN  - 0 (Mutagens)
RN  - 173O8B04RD (hydroxyhydroquinone)
RN  - XV74C1N1AE (hydroquinone)
SB  - IM
MH  - Aneuploidy
MH  - *Chromosome Deletion
MH  - *Chromosomes, Human, Pair 5
MH  - *Chromosomes, Human, Pair 7
MH  - Humans
MH  - Hydroquinones/*pharmacology
MH  - In Situ Hybridization, Fluorescence
MH  - Infant
MH  - Lymphocytes/*drug effects/*physiology
MH  - Mutagens/*pharmacology
EDAT- 1998/05/21 00:00
MHDA- 1998/05/21 00:01
CRDT- 1998/05/21 00:00
PHST- 1998/05/21 00:00 [pubmed]
PHST- 1998/05/21 00:01 [medline]
PHST- 1998/05/21 00:00 [entrez]
AID - S0145212697001574 [pii]
AID - 10.1016/s0145-2126(97)00157-4 [doi]
PST - ppublish
SO  - Leuk Res. 1998 Feb;22(2):105-13. doi: 10.1016/s0145-2126(97)00157-4.