PMID- 9593880
OWN - NLM
STAT- MEDLINE
DCOM- 19980707
LR  - 20190614
IS  - 0006-8993 (Print)
IS  - 0006-8993 (Linking)
VI  - 790
IP  - 1-2
DP  - 1998 Apr 20
TI  - Presynaptic inhibition by noradrenaline of the EPSC evoked in neonatal rat 
      sympathetic preganglionic neurons.
PG  - 170-7
AB  - Visually identified and electrophysiologically characterized sympathetic 
      preganglionic neurons (SPNs) were recorded using the whole-cell voltage clamp 
      technique in slices of neonatal rat spinal cord. Monosynaptic excitatory 
      postsynaptic currents (EPSCs) were evoked by electrical stimulation of the 
      nucleus intercalatus in the presence of strychnine (5 microM) and bicuculline (10 
      microM). These EPSCs were abolished by the antagonist of AMPA-type glutamate 
      receptors, 6-cyano-7-nitro-quinoxaline-2,3-dione (CNQX; 10 microM). Bath applied 
      noradrenaline (NA; 0.5-50 microM) dose-dependently and reversibly decreased by up 
      to around 60% the amplitude of the EPSC, without affecting the holding current. 
      The EPSC depression by NA was not accompanied by a change in EPSC reversal 
      potential (around +5 mV), nor were inward currents generated by pressure 
      application of glutamate affected by NA application. A comparable degree of EPSC 
      depression was also seen with the alpha2-adrenoceptor agonist clonidine (5 
      microM), and the alpha2A-agonist oxymetazoline (5 microM), while the 
      alpha1-agonist phenylephrine (100 microM) caused only a 22% depression. The EPSC 
      depression caused by NA (10 microM) was completely antagonized by either the 
      alpha-antagonist phentolamine (10 microM) or the alpha2-antagonist idazoxan (2 
      microM). Conversely, the beta-adrenoceptor antagonist popranolol (5 microM), and 
      the alpha1-, alpha2B- and alpha2C-antagonist prazosin (2 microM) were without 
      effect. These results indicate that activation of presynaptic 
      alpha2A-adrenoceptors on inputs to SPNs decreases glutamate release.
CI  - Copyright 1998 Elsevier Science B.V.
FAU - Miyazaki, T
AU  - Miyazaki T
AD  - Department of Physiology, Tokyo Medical College, 6-1-1 Shinjuku, Shinjuku-ku, 
      Tokyo 160, Japan. t.miyazaki@bham.ac.uk
FAU - Kobayashi, H
AU  - Kobayashi H
FAU - Tosaka, T
AU  - Tosaka T
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Brain Res
JT  - Brain research
JID - 0045503
RN  - 0 (Adra2a protein, rat)
RN  - 0 (Adrenergic alpha-2 Receptor Agonists)
RN  - 0 (Adrenergic alpha-2 Receptor Antagonists)
RN  - 0 (Adrenergic alpha-Agonists)
RN  - 0 (Adrenergic alpha-Antagonists)
RN  - 0 (Receptors, Adrenergic, alpha-2)
RN  - 1WS297W6MV (Phenylephrine)
RN  - 3KX376GY7L (Glutamic Acid)
RN  - 8VLN5B44ZY (Oxymetazoline)
RN  - MN3L5RMN02 (Clonidine)
RN  - X4W3ENH1CV (Norepinephrine)
RN  - XM03YJ541D (Prazosin)
SB  - IM
MH  - Adrenergic Fibers/drug effects/*physiology
MH  - Adrenergic alpha-2 Receptor Agonists
MH  - Adrenergic alpha-2 Receptor Antagonists
MH  - Adrenergic alpha-Agonists/*pharmacology
MH  - Adrenergic alpha-Antagonists/pharmacology
MH  - Animals
MH  - Animals, Newborn
MH  - Clonidine/pharmacology
MH  - Excitatory Postsynaptic Potentials/*drug effects/physiology
MH  - Glutamic Acid/pharmacology
MH  - Norepinephrine/*pharmacology
MH  - Organ Culture Techniques
MH  - Oxymetazoline/pharmacology
MH  - Patch-Clamp Techniques
MH  - Phenylephrine/pharmacology
MH  - Prazosin/pharmacology
MH  - Presynaptic Terminals/drug effects/*physiology
MH  - Rats
MH  - Receptors, Adrenergic, alpha-2/physiology
MH  - Spinal Cord/chemistry/cytology
EDAT- 1998/06/17 00:00
MHDA- 1998/06/17 00:01
CRDT- 1998/06/17 00:00
PHST- 1998/06/17 00:00 [pubmed]
PHST- 1998/06/17 00:01 [medline]
PHST- 1998/06/17 00:00 [entrez]
AID - S0006-8993(97)01549-7 [pii]
AID - 10.1016/s0006-8993(97)01549-7 [doi]
PST - ppublish
SO  - Brain Res. 1998 Apr 20;790(1-2):170-7. doi: 10.1016/s0006-8993(97)01549-7.