PMID- 9594211
OWN - NLM
STAT- MEDLINE
DCOM- 19980602
LR  - 20061115
IS  - 0366-6999 (Print)
IS  - 0366-6999 (Linking)
VI  - 110
IP  - 7
DP  - 1997 Jul
TI  - Research on the mechanism of endothelin inflammatory effects on human mesangial 
      cells.
PG  - 530-4
AB  - OBJECTIVE: To investigate the mechanism of endothelin (ET) inflammatory effects 
      on human mesangial cells (HMC). METHODS: The following experiments were performed 
      on cultured HMC after ET-1 stimulation: (1) the expression of tumor necrosis 
      factor-alpha (TNF alpha), interleukin-1 beta (IL-1 beta), intercellular adhesion 
      molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1) and endothelin-1 
      (ET-1) itself messenger ribonucleic acid (mRNA) was determined by Northern Blot 
      analysis; (2) the TNF alpha concentration was tested with radioimmunoassay; the 
      IL-1 activity was assayed by the enhancement of thymocyte proliferation in 
      response to mitogen; the surface expression of ICAM-1 and VCAM-1 was measured 
      with cell enzyme linked immunoadsorbent assay (ELISA) analysis. RESULTS: ET-1 
      (10(-7) mol/L) induced the following changes on HMC: (1) up-regulation of the 
      expression of TNF alpha mRNA and protein; (2) up-regulation of the expression of 
      ICAM-1 and VCAM-1 mRNA and protein; (3) up-regulation of the expression of ET-1 
      itself mRNA. However, the expression of IL-1 mRNA and protein was not changed. 
      CONCLUSIONS: ET-1 can stimulate HMC to produce TNF alpha, ICAM-1 and VCAM-1, and 
      thereby induce inflammatory effects. ET-1 can also stimulate HMC to up-regulate 
      the expression of ET-1 itself, so as to amplify inflammatory effects. So, ET-1 is 
      actually an inflammatory mediator and may play an important role in the 
      pathogenesis of glomerulonephritis.
FAU - Chen, Y
AU  - Chen Y
AD  - Division of Nephrology, First Hospital, Beijing Medical University, China.
FAU - Sun, Y
AU  - Sun Y
FAU - Lin, J
AU  - Lin J
FAU - Zhou, A
AU  - Zhou A
FAU - Wang, H
AU  - Wang H
LA  - eng
PT  - Journal Article
PL  - China
TA  - Chin Med J (Engl)
JT  - Chinese medical journal
JID - 7513795
RN  - 0 (Endothelin-1)
RN  - 0 (Interleukin-1)
RN  - 0 (Interleukin-1beta)
RN  - 0 (Interleukin-6)
RN  - 0 (Peptide Fragments)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (Vascular Cell Adhesion Molecule-1)
RN  - 106021-96-9 (interleukin-1beta (163-171))
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
SB  - IM
MH  - Cells, Cultured
MH  - Endothelin-1/metabolism/*pharmacology
MH  - Glomerular Mesangium/cytology/*metabolism
MH  - Humans
MH  - Inflammation
MH  - Intercellular Adhesion Molecule-1/metabolism
MH  - Interleukin-1/*metabolism
MH  - Interleukin-1beta
MH  - Interleukin-6/metabolism
MH  - Peptide Fragments/*metabolism
MH  - Tumor Necrosis Factor-alpha/metabolism
MH  - Up-Regulation
MH  - Vascular Cell Adhesion Molecule-1/metabolism
EDAT- 1997/07/01 00:00
MHDA- 1998/05/22 00:01
CRDT- 1997/07/01 00:00
PHST- 1997/07/01 00:00 [pubmed]
PHST- 1998/05/22 00:01 [medline]
PHST- 1997/07/01 00:00 [entrez]
PST - ppublish
SO  - Chin Med J (Engl). 1997 Jul;110(7):530-4.