PMID- 9595047
OWN - NLM
STAT- MEDLINE
DCOM- 19980602
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 103
IP  - 1
DP  - 1998 May
TI  - A simple efficient method of sequential G-banding and fluorescence in situ 
      hybridization.
PG  - 62-4
AB  - Several methods of chromosome identification to be used in combination with 
      G-banding by the trypsin technique with Giemsa staining (G-banding) and 
      fluorescence in situ hybridization (FISH) have been developed in the past few 
      years. Unfortunately, these methods are impractical to use in the clinical 
      laboratory and can provide inconsistent results. We report a simple, efficient, 
      and reliable method of G-banding and FISH for use in clinical cytogenetics. 
      G-banded chromosomes are codenatured with a direct- or indirect-labeled DNA prove 
      (by using either chromosome painting or a chromosome centromeric probe) followed 
      by a hybridization and signal detection. The procedure requires no pretreatment 
      or additional fixation and results in good preservation of chromosome morphology 
      and good intensity of the FISH signal.
FAU - Zhao, L
AU  - Zhao L
AD  - Section of Clinical Cytogenetics, University of Texas, M. D. Anderson Cancer 
      Center, Houston 77030, USA.
FAU - Hayes, K
AU  - Hayes K
FAU - Glassman, A
AU  - Glassman A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
RN  - 0 (Azure Stains)
RN  - EC 3.4.21.4 (Trypsin)
SB  - IM
MH  - *Azure Stains
MH  - Chromosome Banding/*methods
MH  - Chromosomes, Human/genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Karyotyping
MH  - Microscopy, Fluorescence
MH  - Staining and Labeling/methods
MH  - Trypsin
EDAT- 1998/05/22 00:00
MHDA- 1998/05/22 00:01
CRDT- 1998/05/22 00:00
PHST- 1998/05/22 00:00 [pubmed]
PHST- 1998/05/22 00:01 [medline]
PHST- 1998/05/22 00:00 [entrez]
AID - S0165460897003452 [pii]
AID - 10.1016/s0165-4608(97)00345-2 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 1998 May;103(1):62-4. doi: 10.1016/s0165-4608(97)00345-2.